Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment

Juseok Kim; Joon Yong Kim; Hye Seon Song; Yeon Bee Kim; Tae Woong Whon; Seung Woo Ahn; Se Hee Lee; Seung Ran Yoo; Yu Jin Kim; Jinjong Myoung; Yoon E. Choi; Hong Seok Son; Seong Woon Roh

doi:10.1007/s10482-021-01536-4

Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment

Juseok Kim, Joon Yong Kim, Hye Seon Song, Yeon Bee Kim, Tae Woong Whon, Seung Woo Ahn, Se Hee Lee, Seung Ran Yoo, Yu Jin Kim, Jinjong Myoung, Yoon E. Choi, Hong Seok Son, Seong Woon Roh

Division of Environmental Science and Ecological Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Strain CBA3638^T was isolated from the Geum River sediment, Republic of Korea. The cells of strain CBA3638^T were Gram-stain-positive, strictly anaerobic, rod-shaped, and 0.5–1.0 μm wide, and 4.0–4.5 μm long. Optimal growth occurred at 37 °C, pH 7.0, and 1.0% (w/v) NaCl. Based on the 16S rRNA gene sequence, the phylogenetic analysis showed that strain CBA3638^T belongs to the genus Anaerocolumna in the family Lachnospiraceae, and is most closely related to Anaerocolumna cellulosilytica (94.6–95.0%). The DDH value with A. cellulosilytica SN021^T showed 15.0% relatedness. The genome of strain CBA3638^T consisted of one circular chromosome that is 5,500,435 bp long with a 36.7 mol% G + C content. The genome contained seven 16S-5S-23S rRNA operons and one antibiotic resistance-related transporter gene (mefA). Quinones were not detected. The predominant cellular fatty acids were C_16:0 and C_14:0 and the polar lipids were diphosphatidylglycerol, phosphatidylcholine, and uncharacterised polar lipids. Based on the polyphasic taxonomic analysis, we propose strain CBA3638^T as a novel species in the genus Anaerocolumna, with the name Anaerocolumna sedimenticola sp. nov. The type strain is CBA3638^T (= KACC 21652^T = DSM 110663^T).

Original language	English
Pages (from-to)	507-513
Number of pages	7
Journal	Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume	114
Issue number	5
DOIs	https://doi.org/10.1007/s10482-021-01536-4
Publication status	Published - 2021 May

Keywords

Anaerocolumna sedimenticola
Bacteria
New taxon
The Geum River

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1007/s10482-021-01536-4

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Kim, J., Kim, J. Y., Song, H. S., Kim, Y. B., Whon, T. W., Ahn, S. W., Lee, S. H., Yoo, S. R., Kim, Y. J., Myoung, J., Choi, Y. E., Son, H. S., & Roh, S. W. (2021). Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 114(5), 507-513. https://doi.org/10.1007/s10482-021-01536-4

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title = "Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment",

abstract = "Strain CBA3638T was isolated from the Geum River sediment, Republic of Korea. The cells of strain CBA3638T were Gram-stain-positive, strictly anaerobic, rod-shaped, and 0.5–1.0 μm wide, and 4.0–4.5 μm long. Optimal growth occurred at 37 °C, pH 7.0, and 1.0% (w/v) NaCl. Based on the 16S rRNA gene sequence, the phylogenetic analysis showed that strain CBA3638T belongs to the genus Anaerocolumna in the family Lachnospiraceae, and is most closely related to Anaerocolumna cellulosilytica (94.6–95.0%). The DDH value with A. cellulosilytica SN021T showed 15.0% relatedness. The genome of strain CBA3638T consisted of one circular chromosome that is 5,500,435 bp long with a 36.7 mol% G + C content. The genome contained seven 16S-5S-23S rRNA operons and one antibiotic resistance-related transporter gene (mefA). Quinones were not detected. The predominant cellular fatty acids were C16:0 and C14:0 and the polar lipids were diphosphatidylglycerol, phosphatidylcholine, and uncharacterised polar lipids. Based on the polyphasic taxonomic analysis, we propose strain CBA3638T as a novel species in the genus Anaerocolumna, with the name Anaerocolumna sedimenticola sp. nov. The type strain is CBA3638T (= KACC 21652T = DSM 110663T).",

keywords = "Anaerocolumna sedimenticola, Bacteria, New taxon, The Geum River",

author = "Juseok Kim and Kim, {Joon Yong} and Song, {Hye Seon} and Kim, {Yeon Bee} and Whon, {Tae Woong} and Ahn, {Seung Woo} and Lee, {Se Hee} and Yoo, {Seung Ran} and Kim, {Yu Jin} and Jinjong Myoung and Choi, {Yoon E.} and Son, {Hong Seok} and Roh, {Seong Woon}",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG part of Springer Nature.",

year = "2021",

month = may,

doi = "10.1007/s10482-021-01536-4",

language = "English",

volume = "114",

pages = "507--513",

journal = "Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology",

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T1 - Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment

AU - Kim, Juseok

AU - Kim, Joon Yong

AU - Song, Hye Seon

AU - Kim, Yeon Bee

AU - Whon, Tae Woong

AU - Ahn, Seung Woo

AU - Lee, Se Hee

AU - Yoo, Seung Ran

AU - Kim, Yu Jin

AU - Myoung, Jinjong

AU - Choi, Yoon E.

AU - Son, Hong Seok

AU - Roh, Seong Woon

PY - 2021/5

Y1 - 2021/5

N2 - Strain CBA3638T was isolated from the Geum River sediment, Republic of Korea. The cells of strain CBA3638T were Gram-stain-positive, strictly anaerobic, rod-shaped, and 0.5–1.0 μm wide, and 4.0–4.5 μm long. Optimal growth occurred at 37 °C, pH 7.0, and 1.0% (w/v) NaCl. Based on the 16S rRNA gene sequence, the phylogenetic analysis showed that strain CBA3638T belongs to the genus Anaerocolumna in the family Lachnospiraceae, and is most closely related to Anaerocolumna cellulosilytica (94.6–95.0%). The DDH value with A. cellulosilytica SN021T showed 15.0% relatedness. The genome of strain CBA3638T consisted of one circular chromosome that is 5,500,435 bp long with a 36.7 mol% G + C content. The genome contained seven 16S-5S-23S rRNA operons and one antibiotic resistance-related transporter gene (mefA). Quinones were not detected. The predominant cellular fatty acids were C16:0 and C14:0 and the polar lipids were diphosphatidylglycerol, phosphatidylcholine, and uncharacterised polar lipids. Based on the polyphasic taxonomic analysis, we propose strain CBA3638T as a novel species in the genus Anaerocolumna, with the name Anaerocolumna sedimenticola sp. nov. The type strain is CBA3638T (= KACC 21652T = DSM 110663T).

AB - Strain CBA3638T was isolated from the Geum River sediment, Republic of Korea. The cells of strain CBA3638T were Gram-stain-positive, strictly anaerobic, rod-shaped, and 0.5–1.0 μm wide, and 4.0–4.5 μm long. Optimal growth occurred at 37 °C, pH 7.0, and 1.0% (w/v) NaCl. Based on the 16S rRNA gene sequence, the phylogenetic analysis showed that strain CBA3638T belongs to the genus Anaerocolumna in the family Lachnospiraceae, and is most closely related to Anaerocolumna cellulosilytica (94.6–95.0%). The DDH value with A. cellulosilytica SN021T showed 15.0% relatedness. The genome of strain CBA3638T consisted of one circular chromosome that is 5,500,435 bp long with a 36.7 mol% G + C content. The genome contained seven 16S-5S-23S rRNA operons and one antibiotic resistance-related transporter gene (mefA). Quinones were not detected. The predominant cellular fatty acids were C16:0 and C14:0 and the polar lipids were diphosphatidylglycerol, phosphatidylcholine, and uncharacterised polar lipids. Based on the polyphasic taxonomic analysis, we propose strain CBA3638T as a novel species in the genus Anaerocolumna, with the name Anaerocolumna sedimenticola sp. nov. The type strain is CBA3638T (= KACC 21652T = DSM 110663T).

KW - Anaerocolumna sedimenticola

KW - Bacteria

KW - New taxon

KW - The Geum River

UR - http://www.scopus.com/inward/record.url?scp=85102992075&partnerID=8YFLogxK

U2 - 10.1007/s10482-021-01536-4

DO - 10.1007/s10482-021-01536-4

M3 - Article

C2 - 33730291

AN - SCOPUS:85102992075

SN - 0003-6072

VL - 114

SP - 507

EP - 513

JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

IS - 5

ER -

Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment

Abstract

Keywords

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