Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter

Jaemin An; Jangwon Lee; Youngho Kim; Byungkyu Kim; Sangho Lee

doi:10.1109/NEMS.2008.4484482

Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter

Jaemin An
, Jangwon Lee
, Youngho Kim
, Byungkyu Kim^*
, Sangho Lee

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

9 Citations (Scopus)

Abstract

We investigate the key parameters that influence the efficiency of the previously presented dielectrophoresis(DEP)-activated cell sorter (DACS). While the conventional cell separators typically sort cells depending on the different sizes of cells, our system differentiates similarly-sized cells by responding to the variations of electrical properties among cells namely conductivity and permittivity. Thus, continuously varying DEP forces generated by the system enable us to separate MCF10A (normal cells derived from human breast epithelium) and MCF7 (human breast cancer cells) cells of similar size. Unfortunately, however, both cell types possess a wide range of cell size variation. In previous study, therefore, separation efficiency was limited to 77±5% for sorting MCF10A cells and MCF7 cells. To analyze separation efficiency according to cell size, we calculate cell size distributions before and after separation and then analyze the separation efficiency of cells according to cell size. Although cell separation is performed relying on the electrical properties of the cell, we conclude that cell size remains one of the key parameter that determines the efficiency of cell separation.

Original language	English
Title of host publication	3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
Publisher	IEEE Computer Society
Pages	965-969
Number of pages	5
ISBN (Print)	9781424419081
DOIs	https://doi.org/10.1109/NEMS.2008.4484482
Publication status	Published - 2008
Event	3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 - Sanya, China Duration: 2008 Jan 6 → 2008 Jan 9

Publication series

Name	3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS

Other

Other	3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
Country/Territory	China
City	Sanya
Period	08/1/6 → 08/1/9

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 7 Affordable and Clean Energy

Keywords

Conductivity
DACS
Dielectrophoresis(DEP)
MCF10A
MCF7
Permittivity

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment
Management, Monitoring, Policy and Law
General Energy
Energy Engineering and Power Technology
Modelling and Simulation
Fuel Technology
Control and Systems Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/NEMS.2008.4484482

Cite this

An, J., Lee, J., Kim, Y., Kim, B., & Lee, S. (2008). Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter. In 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 (pp. 965-969). Article 4484482 (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS). IEEE Computer Society. https://doi.org/10.1109/NEMS.2008.4484482

Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter. / An, Jaemin; Lee, Jangwon; Kim, Youngho et al.
3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008. IEEE Computer Society, 2008. p. 965-969 4484482 (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

An, J, Lee, J, Kim, Y, Kim, B & Lee, S 2008, Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter. in 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008., 4484482, 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS, IEEE Computer Society, pp. 965-969, 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008, Sanya, China, 08/1/6. https://doi.org/10.1109/NEMS.2008.4484482

An J, Lee J, Kim Y, Kim B, Lee S. Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter. In 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008. IEEE Computer Society. 2008. p. 965-969. 4484482. (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS). doi: 10.1109/NEMS.2008.4484482

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title = "Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter",

abstract = "We investigate the key parameters that influence the efficiency of the previously presented dielectrophoresis(DEP)-activated cell sorter (DACS). While the conventional cell separators typically sort cells depending on the different sizes of cells, our system differentiates similarly-sized cells by responding to the variations of electrical properties among cells namely conductivity and permittivity. Thus, continuously varying DEP forces generated by the system enable us to separate MCF10A (normal cells derived from human breast epithelium) and MCF7 (human breast cancer cells) cells of similar size. Unfortunately, however, both cell types possess a wide range of cell size variation. In previous study, therefore, separation efficiency was limited to 77±5\% for sorting MCF10A cells and MCF7 cells. To analyze separation efficiency according to cell size, we calculate cell size distributions before and after separation and then analyze the separation efficiency of cells according to cell size. Although cell separation is performed relying on the electrical properties of the cell, we conclude that cell size remains one of the key parameter that determines the efficiency of cell separation.",

keywords = "Conductivity, DACS, Dielectrophoresis(DEP), MCF10A, MCF7, Permittivity",

author = "Jaemin An and Jangwon Lee and Youngho Kim and Byungkyu Kim and Sangho Lee",

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doi = "10.1109/NEMS.2008.4484482",

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AU - An, Jaemin

AU - Lee, Jangwon

AU - Kim, Youngho

AU - Kim, Byungkyu

AU - Lee, Sangho

PY - 2008

Y1 - 2008

N2 - We investigate the key parameters that influence the efficiency of the previously presented dielectrophoresis(DEP)-activated cell sorter (DACS). While the conventional cell separators typically sort cells depending on the different sizes of cells, our system differentiates similarly-sized cells by responding to the variations of electrical properties among cells namely conductivity and permittivity. Thus, continuously varying DEP forces generated by the system enable us to separate MCF10A (normal cells derived from human breast epithelium) and MCF7 (human breast cancer cells) cells of similar size. Unfortunately, however, both cell types possess a wide range of cell size variation. In previous study, therefore, separation efficiency was limited to 77±5% for sorting MCF10A cells and MCF7 cells. To analyze separation efficiency according to cell size, we calculate cell size distributions before and after separation and then analyze the separation efficiency of cells according to cell size. Although cell separation is performed relying on the electrical properties of the cell, we conclude that cell size remains one of the key parameter that determines the efficiency of cell separation.

AB - We investigate the key parameters that influence the efficiency of the previously presented dielectrophoresis(DEP)-activated cell sorter (DACS). While the conventional cell separators typically sort cells depending on the different sizes of cells, our system differentiates similarly-sized cells by responding to the variations of electrical properties among cells namely conductivity and permittivity. Thus, continuously varying DEP forces generated by the system enable us to separate MCF10A (normal cells derived from human breast epithelium) and MCF7 (human breast cancer cells) cells of similar size. Unfortunately, however, both cell types possess a wide range of cell size variation. In previous study, therefore, separation efficiency was limited to 77±5% for sorting MCF10A cells and MCF7 cells. To analyze separation efficiency according to cell size, we calculate cell size distributions before and after separation and then analyze the separation efficiency of cells according to cell size. Although cell separation is performed relying on the electrical properties of the cell, we conclude that cell size remains one of the key parameter that determines the efficiency of cell separation.

KW - Conductivity

KW - DACS

KW - Dielectrophoresis(DEP)

KW - MCF10A

KW - MCF7

KW - Permittivity

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M3 - Conference contribution

AN - SCOPUS:50249101709

SN - 9781424419081

T3 - 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS

SP - 965

EP - 969

BT - 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008

PB - IEEE Computer Society

T2 - 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008

Y2 - 6 January 2008 through 9 January 2008

ER -

Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter

Abstract

Publication series

Other

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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