Analysis of differentially regulated proteins in TM4 cells treated with bisphenol A

Do Youn Lee; Sang Soo Lee; Won A. Joo; Eun Ju Lee; Chan Wha Kim

doi:10.1271/bbb.68.1201

Analysis of differentially regulated proteins in TM4 cells treated with bisphenol A

Do Youn Lee, Sang Soo Lee, Won A. Joo, Eun Ju Lee, Chan Wha Kim

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

BPA, bisphenol A, a monomer of epoxy resins and polycarbonate plastic, is used in many consumer products including the plastic linings of cans for food and babies' bottles. BPA has been reported to cause reproductive toxicity and affects cells in rats and mice at high doses. In this study, the effect of BPA on protein expression in TM4 cells (a mouse Sertoli cell line) known to play an essential role in Spermatogenesis was investigated by two-dimensional electrophoresis (2-DE). After 16 h exposure to 50, 100, 150, 200, and 250 μM of BPA, the viability of TM4 cells decreased to about 90, 85, 78, 55, and 30% of control respectively. Approximately 800 protein spots in TM4 cells were analyzed by 2-DE with pH 4-7 linear immobilized pH gradient (IPG) Dry Strip, and 11 proteins which showed significantly different expression levels were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Among these, HSP 27 and placental calcium binding protein may be proteins differentially expressed by BPA exposure.

Original language	English
Pages (from-to)	1201-1208
Number of pages	8
Journal	Bioscience, Biotechnology and Biochemistry
Volume	68
Issue number	6
DOIs	https://doi.org/10.1271/bbb.68.1201
Publication status	Published - 2004 Jun

Bibliographical note

Funding Information:
This study was supported by the Korean Ministry of Commerce, Industy & Energy. Sang-Soo Lee held a Brain Korea 21 fellowship from the Korean Ministry of Education. We thank Seo Jong-Bok of the Korea Basic Science Institute for his support in the MALDI-TOF MS analysis.

Keywords

2D gel electrohphoresis
Bisphenol A
Calcium binding protein
HSP 27
Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDITOF MS)
TM 4 cell

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Analysis of differentially regulated proteins in TM4 cells treated with bisphenol A",

abstract = "BPA, bisphenol A, a monomer of epoxy resins and polycarbonate plastic, is used in many consumer products including the plastic linings of cans for food and babies' bottles. BPA has been reported to cause reproductive toxicity and affects cells in rats and mice at high doses. In this study, the effect of BPA on protein expression in TM4 cells (a mouse Sertoli cell line) known to play an essential role in Spermatogenesis was investigated by two-dimensional electrophoresis (2-DE). After 16 h exposure to 50, 100, 150, 200, and 250 μM of BPA, the viability of TM4 cells decreased to about 90, 85, 78, 55, and 30% of control respectively. Approximately 800 protein spots in TM4 cells were analyzed by 2-DE with pH 4-7 linear immobilized pH gradient (IPG) Dry Strip, and 11 proteins which showed significantly different expression levels were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Among these, HSP 27 and placental calcium binding protein may be proteins differentially expressed by BPA exposure.",

keywords = "2D gel electrohphoresis, Bisphenol A, Calcium binding protein, HSP 27, Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDITOF MS), TM 4 cell",

author = "Lee, {Do Youn} and Lee, {Sang Soo} and Joo, {Won A.} and Lee, {Eun Ju} and Kim, {Chan Wha}",

note = "Funding Information: This study was supported by the Korean Ministry of Commerce, Industy & Energy. Sang-Soo Lee held a Brain Korea 21 fellowship from the Korean Ministry of Education. We thank Seo Jong-Bok of the Korea Basic Science Institute for his support in the MALDI-TOF MS analysis.",

year = "2004",

month = jun,

doi = "10.1271/bbb.68.1201",

language = "English",

volume = "68",

pages = "1201--1208",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Oxford University Press",

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AU - Lee, Do Youn

AU - Lee, Sang Soo

AU - Joo, Won A.

AU - Lee, Eun Ju

AU - Kim, Chan Wha

N1 - Funding Information: This study was supported by the Korean Ministry of Commerce, Industy & Energy. Sang-Soo Lee held a Brain Korea 21 fellowship from the Korean Ministry of Education. We thank Seo Jong-Bok of the Korea Basic Science Institute for his support in the MALDI-TOF MS analysis.

PY - 2004/6

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N2 - BPA, bisphenol A, a monomer of epoxy resins and polycarbonate plastic, is used in many consumer products including the plastic linings of cans for food and babies' bottles. BPA has been reported to cause reproductive toxicity and affects cells in rats and mice at high doses. In this study, the effect of BPA on protein expression in TM4 cells (a mouse Sertoli cell line) known to play an essential role in Spermatogenesis was investigated by two-dimensional electrophoresis (2-DE). After 16 h exposure to 50, 100, 150, 200, and 250 μM of BPA, the viability of TM4 cells decreased to about 90, 85, 78, 55, and 30% of control respectively. Approximately 800 protein spots in TM4 cells were analyzed by 2-DE with pH 4-7 linear immobilized pH gradient (IPG) Dry Strip, and 11 proteins which showed significantly different expression levels were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Among these, HSP 27 and placental calcium binding protein may be proteins differentially expressed by BPA exposure.

AB - BPA, bisphenol A, a monomer of epoxy resins and polycarbonate plastic, is used in many consumer products including the plastic linings of cans for food and babies' bottles. BPA has been reported to cause reproductive toxicity and affects cells in rats and mice at high doses. In this study, the effect of BPA on protein expression in TM4 cells (a mouse Sertoli cell line) known to play an essential role in Spermatogenesis was investigated by two-dimensional electrophoresis (2-DE). After 16 h exposure to 50, 100, 150, 200, and 250 μM of BPA, the viability of TM4 cells decreased to about 90, 85, 78, 55, and 30% of control respectively. Approximately 800 protein spots in TM4 cells were analyzed by 2-DE with pH 4-7 linear immobilized pH gradient (IPG) Dry Strip, and 11 proteins which showed significantly different expression levels were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Among these, HSP 27 and placental calcium binding protein may be proteins differentially expressed by BPA exposure.

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Analysis of differentially regulated proteins in TM4 cells treated with bisphenol A

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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