Analysis of single nucleotide polymorphism among Varicella-Zoster Virus and identification of vaccine-specific sites

Jeong Seon Jeon, Youn Hee Won, In Kyo Kim, Jin Hyun Ahn, Ok Sarah Shin, Jung Hwan Kim, Chan Hee Lee

    Research output: Contribution to journalArticlepeer-review

    37 Citations (Scopus)

    Abstract

    Varicella-zoster virus (VZV) is a causative agent for chickenpox and zoster. Live attenuated vaccines have been developed based on Oka and MAV/06 strains. In order to understand the molecular mechanisms of attenuation, complete genome sequences of vaccine and wild-type strains were compared and single nucleotide polymorphism (SNP) was analyzed. ORF22 and ORF62 contained the highest number of SNPs. The detailed analysis of the SNPs suggested 24 potential vaccine-specific sites. All the mutational events found in vaccine-specific sites were transitional, and most of them were substitution of AT to GC pair. Interestingly, 18 of the vaccine-specific sites of the vaccine strains appeared to be genetically heterogeneous. The probability of a single genome of vaccine strain to contain all 24 vaccine-type sequences was calculated to be less than 4%. The average codon adaptation index (CAI) value of the vaccine strains was significantly lower than the CAI value of the clinical strains.

    Original languageEnglish
    Pages (from-to)277-286
    Number of pages10
    JournalVirology
    Volume496
    DOIs
    Publication statusPublished - 2016 Sept 1

    Bibliographical note

    Publisher Copyright:
    © 2016 Elsevier Inc.

    Keywords

    • Genetic heterogeneity
    • Live attenuated vaccine
    • Sequence diversity
    • Single nucleotide polymorphism (SNP)
    • Vaccine-specific sites
    • Varicella-zoster virus (VZV)

    ASJC Scopus subject areas

    • Virology

    Fingerprint

    Dive into the research topics of 'Analysis of single nucleotide polymorphism among Varicella-Zoster Virus and identification of vaccine-specific sites'. Together they form a unique fingerprint.

    Cite this