Analysis of the immune-inducible genes of Plutella xylostella using expressed sequence tags and cDNA microarray

Jai Hoon Eum, Young Rok Seo, Sung Moon Yoe, Seok Woo Kang, Sung Sik Han

    Research output: Contribution to journalArticlepeer-review

    36 Citations (Scopus)

    Abstract

    In the present study, the complex gene expression responses of Plutella xylostella to microbial challenges and injury were surveyed using a newly constructed expressed sequence tag (EST) clone collection and cDNA microarray analysis. A total of 1132 P. xylostella ESTs were cloned, annotated and categorized by their putative functions; these included proteases, protease inhibitors, recognition molecules and anti-microbial peptides. GeneOntology revealed that 4% of the P. xylostella ESTs corresponded to immunity-related genes potentially involved in innate immunity. We then used microarray analysis to identify 44 genes that were differentially expressed with at least a two-fold expression difference in P. xylostella before and after pathogen challenge. Together, our EST categorization and microarray profiling analyses allowed us to identify 70 genes that should be considered candidate immune response genes, providing important new insights into the molecular events that occur during the innate immune response in P. xylostella.

    Original languageEnglish
    Pages (from-to)1107-1120
    Number of pages14
    JournalDevelopmental and Comparative Immunology
    Volume31
    Issue number11
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Expressed sequence tags (ESTs)
    • Immune response
    • Microarray
    • Plutella xylostella
    • pxCecropin E (pxCecE)
    • pxS-adenosyl-l-homocysteine hydrolase (pxAhh)

    ASJC Scopus subject areas

    • Immunology
    • Developmental Biology

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