TY - GEN
T1 - Analysis of the production of L929 cell-based cytokine, M-CSF, using 3D spheroid culture system
AU - Jung, Yong Hun
AU - Na, Kyuhwan
AU - Kim, Jeahoon
AU - Choi, Donghee
AU - Kim, Hyunho
AU - Cho, Youngkyu
AU - Chung, Seok
PY - 2020/1/1
Y1 - 2020/1/1
N2 - A mouse fibroblast cell line, L929, produces the Macrophage colony-stimulating factor(M-csf) which is a hematopoietic growth factor that plays a critical role in the survival and differentiation of hematopoietic cell lineage.[1] In order to supply sufficient M-csf, three conventional methods are available: co-culturing fibroblast, purchasing recombinant M-csf, and applying M-csf conditioned media. However, conventional methods based on 2-D possess severe drawbacks in space limitation, long term culture, and inconsistent M-csf production. To overcome such inefficiency, we introduce simple spheroid based M-csf harvesting method. Comparing with 2-D data, spheroids numbered at 361 ensure space control, long term culture and M-csf production.
AB - A mouse fibroblast cell line, L929, produces the Macrophage colony-stimulating factor(M-csf) which is a hematopoietic growth factor that plays a critical role in the survival and differentiation of hematopoietic cell lineage.[1] In order to supply sufficient M-csf, three conventional methods are available: co-culturing fibroblast, purchasing recombinant M-csf, and applying M-csf conditioned media. However, conventional methods based on 2-D possess severe drawbacks in space limitation, long term culture, and inconsistent M-csf production. To overcome such inefficiency, we introduce simple spheroid based M-csf harvesting method. Comparing with 2-D data, spheroids numbered at 361 ensure space control, long term culture and M-csf production.
KW - Efficient
KW - Fibroblast
KW - Macrophage Colony-Stimulating Factor
KW - Production
KW - Spheroid
UR - http://www.scopus.com/inward/record.url?scp=85079614575&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079614575&partnerID=8YFLogxK
M3 - Conference contribution
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 999
EP - 1000
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -