Analysis of the stress effects of endocrine disrupting chemicals (EDCs) on Escherichia coli

Yeon Seok Kim; Jiho Min; Han Na Hong; Ji Hyun Park; Kyeong Seo Park; Man Bock Gu

Analysis of the stress effects of endocrine disrupting chemicals (EDCs) on Escherichia coli

Yeon Seok Kim, Jiho Min, Han Na Hong, Ji Hyun Park, Kyeong Seo Park, Man Bock Gu

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, three of the representative EDCs, 17β-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas 17β-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.

Original language	English
Pages (from-to)	1390-1393
Number of pages	4
Journal	Journal of microbiology and biotechnology
Volume	17
Issue number	8
Publication status	Published - 2007 Aug

Keywords

Endocrine disrupting chemicals (EDCs)
Gene expression analysis
Real-time RT-PCR
Stress effect
Stress-specific responsive gene

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

Cite this

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title = "Analysis of the stress effects of endocrine disrupting chemicals (EDCs) on Escherichia coli",

abstract = "In this study, three of the representative EDCs, 17β-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas 17β-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.",

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author = "Kim, {Yeon Seok} and Jiho Min and Hong, {Han Na} and Park, {Ji Hyun} and Park, {Kyeong Seo} and Gu, {Man Bock}",

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T1 - Analysis of the stress effects of endocrine disrupting chemicals (EDCs) on Escherichia coli

AU - Kim, Yeon Seok

AU - Min, Jiho

AU - Hong, Han Na

AU - Park, Ji Hyun

AU - Park, Kyeong Seo

AU - Gu, Man Bock

PY - 2007/8

Y1 - 2007/8

N2 - In this study, three of the representative EDCs, 17β-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas 17β-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.

AB - In this study, three of the representative EDCs, 17β-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas 17β-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.

KW - Endocrine disrupting chemicals (EDCs)

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KW - Real-time RT-PCR

KW - Stress effect

KW - Stress-specific responsive gene

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Analysis of the stress effects of endocrine disrupting chemicals (EDCs) on Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

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