Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo

Jungsu Hwang; Chan Woo Kim; Kyung No Son; Kyu Yeon Han; Kyung Hee Lee; Hynda K. Kleinman; Jesang Ko; Doe Sun Na; Byoung S. Kwon; Yong Song Gho; Jiyoung Kim

doi:10.1016/j.febslet.2004.06.023

Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo

Jungsu Hwang
, Chan Woo Kim
, Kyung No Son
, Kyu Yeon Han
, Kyung Hee Lee
, Hynda K. Kleinman
, Jesang Ko
, Doe Sun Na
, Byoung S. Kwon
, Yong Song Gho^*
, Jiyoung Kim

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

Abstract

CCL15 is a novel human CC chemokine and exerts its biological activities on immune cells through CCR1 and CCR3. Because a number of chemokines induce angiogenesis and endothelial cells express CCR1 and CCR3, we investigated the angiogenic activity of CCL15. Both CCL15(1-92) and N-terminal truncated CCL15(25-92) stimulate the chemotactic endothelial cell migration and differentiation, but CCL15(25-92) is at least 100-fold more potent than CCL15(1-92). Treatment with pertussis toxin (PTX), with anti-CCR1, or with anti-CCR3 antibody inhibits the CCL15(25-92)-induced endothelial cell migration. CCL15(25-92) also stimulates sprouting of vessels from aortic rings and mediates angiogenesis in the chick chorioallantoic membrane assay. Our findings demonstrate that CCL15(25-92) has in vitro and in vivo angiogenic activity, and suggest roles of the chemokine in angiogenesis.

Original language	English
Pages (from-to)	47-51
Number of pages	5
Journal	FEBS Letters
Volume	570
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.06.023
Publication status	Published - 2004 Jul 16
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by an Immunomodulation Research Center grant (to J.K.) from KOSEF and a Vascular System Research Center grant (to Y.S.G.). J.H., K.N.S. and K.H.L. were supported by BK21 program of Ministry of Education.

Keywords

Angiogenesis
CCL15
CCR1
CCR3
Chemokine
Endothelial cells

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2004.06.023

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title = "Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo",

abstract = "CCL15 is a novel human CC chemokine and exerts its biological activities on immune cells through CCR1 and CCR3. Because a number of chemokines induce angiogenesis and endothelial cells express CCR1 and CCR3, we investigated the angiogenic activity of CCL15. Both CCL15(1-92) and N-terminal truncated CCL15(25-92) stimulate the chemotactic endothelial cell migration and differentiation, but CCL15(25-92) is at least 100-fold more potent than CCL15(1-92). Treatment with pertussis toxin (PTX), with anti-CCR1, or with anti-CCR3 antibody inhibits the CCL15(25-92)-induced endothelial cell migration. CCL15(25-92) also stimulates sprouting of vessels from aortic rings and mediates angiogenesis in the chick chorioallantoic membrane assay. Our findings demonstrate that CCL15(25-92) has in vitro and in vivo angiogenic activity, and suggest roles of the chemokine in angiogenesis.",

keywords = "Angiogenesis, CCL15, CCR1, CCR3, Chemokine, Endothelial cells",

author = "Jungsu Hwang and Kim, \{Chan Woo\} and Son, \{Kyung No\} and Han, \{Kyu Yeon\} and Lee, \{Kyung Hee\} and Kleinman, \{Hynda K.\} and Jesang Ko and Na, \{Doe Sun\} and Kwon, \{Byoung S.\} and Gho, \{Yong Song\} and Jiyoung Kim",

note = "Funding Information: This work was supported by an Immunomodulation Research Center grant (to J.K.) from KOSEF and a Vascular System Research Center grant (to Y.S.G.). J.H., K.N.S. and K.H.L. were supported by BK21 program of Ministry of Education.",

year = "2004",

month = jul,

day = "16",

doi = "10.1016/j.febslet.2004.06.023",

language = "English",

volume = "570",

pages = "47--51",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "1-3",

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TY - JOUR

T1 - Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo

AU - Hwang, Jungsu

AU - Kim, Chan Woo

AU - Son, Kyung No

AU - Han, Kyu Yeon

AU - Lee, Kyung Hee

AU - Kleinman, Hynda K.

AU - Ko, Jesang

AU - Na, Doe Sun

AU - Kwon, Byoung S.

AU - Gho, Yong Song

AU - Kim, Jiyoung

N1 - Funding Information: This work was supported by an Immunomodulation Research Center grant (to J.K.) from KOSEF and a Vascular System Research Center grant (to Y.S.G.). J.H., K.N.S. and K.H.L. were supported by BK21 program of Ministry of Education.

PY - 2004/7/16

Y1 - 2004/7/16

N2 - CCL15 is a novel human CC chemokine and exerts its biological activities on immune cells through CCR1 and CCR3. Because a number of chemokines induce angiogenesis and endothelial cells express CCR1 and CCR3, we investigated the angiogenic activity of CCL15. Both CCL15(1-92) and N-terminal truncated CCL15(25-92) stimulate the chemotactic endothelial cell migration and differentiation, but CCL15(25-92) is at least 100-fold more potent than CCL15(1-92). Treatment with pertussis toxin (PTX), with anti-CCR1, or with anti-CCR3 antibody inhibits the CCL15(25-92)-induced endothelial cell migration. CCL15(25-92) also stimulates sprouting of vessels from aortic rings and mediates angiogenesis in the chick chorioallantoic membrane assay. Our findings demonstrate that CCL15(25-92) has in vitro and in vivo angiogenic activity, and suggest roles of the chemokine in angiogenesis.

AB - CCL15 is a novel human CC chemokine and exerts its biological activities on immune cells through CCR1 and CCR3. Because a number of chemokines induce angiogenesis and endothelial cells express CCR1 and CCR3, we investigated the angiogenic activity of CCL15. Both CCL15(1-92) and N-terminal truncated CCL15(25-92) stimulate the chemotactic endothelial cell migration and differentiation, but CCL15(25-92) is at least 100-fold more potent than CCL15(1-92). Treatment with pertussis toxin (PTX), with anti-CCR1, or with anti-CCR3 antibody inhibits the CCL15(25-92)-induced endothelial cell migration. CCL15(25-92) also stimulates sprouting of vessels from aortic rings and mediates angiogenesis in the chick chorioallantoic membrane assay. Our findings demonstrate that CCL15(25-92) has in vitro and in vivo angiogenic activity, and suggest roles of the chemokine in angiogenesis.

KW - Angiogenesis

KW - CCL15

KW - CCR1

KW - CCR3

KW - Chemokine

KW - Endothelial cells

UR - https://www.scopus.com/pages/publications/3142636528

U2 - 10.1016/j.febslet.2004.06.023

DO - 10.1016/j.febslet.2004.06.023

M3 - Article

C2 - 15251437

AN - SCOPUS:3142636528

SN - 0014-5793

VL - 570

SP - 47

EP - 51

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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