Anisotropic CdSe Tetrapods in Vortex Flow for Removing Non-Specific Binding and Increasing Protein Capture

Hanzhe Liu, Dong June Ahn

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Non-specific binding (NSB) is one of the important issues in biosensing performance. Herein, we designed a strategy for removing non-specific binding including anti-mouse IgG antibody and bovine serum albumin (BSA) by utilizing anisotropic cadmium selenide tetrapods (CdSe TPs) in a vortex flow. The shear force on the tetrapod nanoparticles was increased by controlling the rotation rate of the vortex flow from 0 rpm to 1000 rpm. As a result, photoluminescence (PL) signals of fluorescein (FITC)-conjugated protein, anti-mouse IgG antibody-FITC and bovine serum albumin (BSA)-FITC, were reduced by 35% and 45%, respectively, indicating that NSB can be removed under vortex flow. In particular, simultaneous NSB removal and protein capture can be achieved even with mixture solutions of target antibodies and anti-mouse IgG antibodies by applying cyclic mode vortex flow on anisotropic CdSe TPs. These results demonstrate successfully that NSB can be diminished by rotating CdSe TPs to generate shear force under vortex flow. This study opens up new research protocols for utilization of anisotropic nanoparticles under vortex flow, which increases the feasibility of protein capture and non-specific proteins removal for biosensors.

Original languageEnglish
Article number5929
Issue number15
Publication statusPublished - 2022 Aug

Bibliographical note

Funding Information:
This work supported by National Research Foundation of Korea (NRF-2021R1A2C3009955), and a Korea University Grant.

Publisher Copyright:
© 2022 by the authors.


  • CdSe tetrapods
  • anisotropic nanoparticles
  • cyclic mode vortex flow
  • non-specific binding removal
  • protein capture

ASJC Scopus subject areas

  • Analytical Chemistry
  • Information Systems
  • Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Electrical and Electronic Engineering


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