Abstract
We studied the interaction between cAMP and protein kinase C (PKC) signaling pathways in the regulation of fibronectin synthesis in human lung fibroblasts. Phorbol myristate acetate (PMA), a PKC activator, stimulated fibronectin synthesis and its mRNA expression in both normal and transformed human lung fibroblasts (WI-38 and WI-38 VA13, respectively). On the other hand, dibutyryl cAMP (Bt2cAMP), a cAMP analogue, did not alter fibronectin synthesis in both cell lines. The combined treatment of Bt2cAMP with PMA, however, suppressed the PMA-induced stimulation of fibronectin synthesis and mRNA expression in these cells. This study shows that cAMP pathway antagonizes PKC pathway in regulating fibronectin synthesis in human lung fibroblasts and provides an example of antagonistic interaction between cAMP and PKC signaling pathways.
Original language | English |
---|---|
Pages (from-to) | 383-390 |
Number of pages | 8 |
Journal | Biochemistry and Molecular Biology International |
Volume | 43 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1997 Oct |
Externally published | Yes |
Keywords
- Cross-talk
- Fibroblasts
- Fibronectin
- Protein kinase C
- cAMP
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics