Anti-CD3 single-chain Fv/interleukin-18 fusion DNA induces anti-mycobacterial resistance via efficient interferon-γ production in BALB/c mice infected with Mycobacterium avium

Seung H. Kim; Daeho Cho; Tae S. Kim

doi:10.1016/j.vaccine.2005.12.065

Anti-CD3 single-chain Fv/interleukin-18 fusion DNA induces anti-mycobacterial resistance via efficient interferon-γ production in BALB/c mice infected with Mycobacterium avium

Seung H. Kim, Daeho Cho, Tae S. Kim

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

T helper type 1 (Th1) cell-mediated immune response performs a critical role in the induction of host defenses against a variety of intracellular pathogens. We recently demonstrated that the linkage between interleukin (IL)-18 and anti-CD3sFv genes confined the effect of IL-18 to the CD3⁺ T cells, resulting in the efficient induction of Thl immune responses. In this study, we attempted to determine whether the anti-CD3 single-chain Fv/IL-18 fusion DNA (pAnti-CD3sFv/IL-18) could stimulate resistance to Mycobacterium avium infection in genetically susceptible BALB/c mice. Immunization with pAnti-CD3sFv/IL-18 DNA during intranasal infection with M. avium resulted in a significant reduction in the bacterial load in the lung throughout the entire 10-week observation period. Immunization with pAnti-CD3sFv/IL-18 DNA induced and maintained significantly higher levels of cytotoxic activity and nitric oxide production by the lung cells, as compared with the injection of pAnti-CD3sFv DNA, or of a simple mixture of pAnti-CD3sFv DNA and pIL-18 DNA. Furthermore, the lung cells of the mice injected with pAnti-CD3sFv/IL-18 DNA exhibited persistent production of IFN-γ. Treatment with anti-IFN-γ antibody resulted in an abrogation of anti-mycobacterial effects in mice injected with pAnti-CD3sFv/IL-18 DNA. These results indicate that vaccination with the pAnti-CD3sFv/IL-18 fusion DNA induces significant resistance to M. avium infection, via the production of IFN-γ in CD4⁺ T cells.

Original language	English
Pages (from-to)	3365-3373
Number of pages	9
Journal	Vaccine
Volume	24
Issue number	16
DOIs	https://doi.org/10.1016/j.vaccine.2005.12.065
Publication status	Published - 2006 Apr 12

Keywords

CD3 T cell
Fusion DNA
IL-18
Immunity
Mycobacterium avium

ASJC Scopus subject areas

Molecular Medicine
Immunology and Microbiology(all)
veterinary(all)
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.vaccine.2005.12.065

Cite this

@article{af747402cf9d4008b03a07b7ed050543,

title = "Anti-CD3 single-chain Fv/interleukin-18 fusion DNA induces anti-mycobacterial resistance via efficient interferon-γ production in BALB/c mice infected with Mycobacterium avium",

abstract = "T helper type 1 (Th1) cell-mediated immune response performs a critical role in the induction of host defenses against a variety of intracellular pathogens. We recently demonstrated that the linkage between interleukin (IL)-18 and anti-CD3sFv genes confined the effect of IL-18 to the CD3+ T cells, resulting in the efficient induction of Thl immune responses. In this study, we attempted to determine whether the anti-CD3 single-chain Fv/IL-18 fusion DNA (pAnti-CD3sFv/IL-18) could stimulate resistance to Mycobacterium avium infection in genetically susceptible BALB/c mice. Immunization with pAnti-CD3sFv/IL-18 DNA during intranasal infection with M. avium resulted in a significant reduction in the bacterial load in the lung throughout the entire 10-week observation period. Immunization with pAnti-CD3sFv/IL-18 DNA induced and maintained significantly higher levels of cytotoxic activity and nitric oxide production by the lung cells, as compared with the injection of pAnti-CD3sFv DNA, or of a simple mixture of pAnti-CD3sFv DNA and pIL-18 DNA. Furthermore, the lung cells of the mice injected with pAnti-CD3sFv/IL-18 DNA exhibited persistent production of IFN-γ. Treatment with anti-IFN-γ antibody resulted in an abrogation of anti-mycobacterial effects in mice injected with pAnti-CD3sFv/IL-18 DNA. These results indicate that vaccination with the pAnti-CD3sFv/IL-18 fusion DNA induces significant resistance to M. avium infection, via the production of IFN-γ in CD4+ T cells.",

keywords = "CD3 T cell, Fusion DNA, IL-18, Immunity, Mycobacterium avium",

author = "Kim, {Seung H.} and Daeho Cho and Kim, {Tae S.}",

note = "Funding Information: We would like to thank Dr. D.S. Lim, Dr. B.R. Blazar, and Dr. M.E. Reff for providing valuable reagents. This study was supported by a grant of the SRC/ERC program of the KOSEF/MOST (R11-2005-017-02001-0). ",

year = "2006",

month = apr,

day = "12",

doi = "10.1016/j.vaccine.2005.12.065",

language = "English",

volume = "24",

pages = "3365--3373",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

number = "16",

}

TY - JOUR

T1 - Anti-CD3 single-chain Fv/interleukin-18 fusion DNA induces anti-mycobacterial resistance via efficient interferon-γ production in BALB/c mice infected with Mycobacterium avium

AU - Kim, Seung H.

AU - Cho, Daeho

AU - Kim, Tae S.

N1 - Funding Information: We would like to thank Dr. D.S. Lim, Dr. B.R. Blazar, and Dr. M.E. Reff for providing valuable reagents. This study was supported by a grant of the SRC/ERC program of the KOSEF/MOST (R11-2005-017-02001-0).

PY - 2006/4/12

Y1 - 2006/4/12

N2 - T helper type 1 (Th1) cell-mediated immune response performs a critical role in the induction of host defenses against a variety of intracellular pathogens. We recently demonstrated that the linkage between interleukin (IL)-18 and anti-CD3sFv genes confined the effect of IL-18 to the CD3+ T cells, resulting in the efficient induction of Thl immune responses. In this study, we attempted to determine whether the anti-CD3 single-chain Fv/IL-18 fusion DNA (pAnti-CD3sFv/IL-18) could stimulate resistance to Mycobacterium avium infection in genetically susceptible BALB/c mice. Immunization with pAnti-CD3sFv/IL-18 DNA during intranasal infection with M. avium resulted in a significant reduction in the bacterial load in the lung throughout the entire 10-week observation period. Immunization with pAnti-CD3sFv/IL-18 DNA induced and maintained significantly higher levels of cytotoxic activity and nitric oxide production by the lung cells, as compared with the injection of pAnti-CD3sFv DNA, or of a simple mixture of pAnti-CD3sFv DNA and pIL-18 DNA. Furthermore, the lung cells of the mice injected with pAnti-CD3sFv/IL-18 DNA exhibited persistent production of IFN-γ. Treatment with anti-IFN-γ antibody resulted in an abrogation of anti-mycobacterial effects in mice injected with pAnti-CD3sFv/IL-18 DNA. These results indicate that vaccination with the pAnti-CD3sFv/IL-18 fusion DNA induces significant resistance to M. avium infection, via the production of IFN-γ in CD4+ T cells.

AB - T helper type 1 (Th1) cell-mediated immune response performs a critical role in the induction of host defenses against a variety of intracellular pathogens. We recently demonstrated that the linkage between interleukin (IL)-18 and anti-CD3sFv genes confined the effect of IL-18 to the CD3+ T cells, resulting in the efficient induction of Thl immune responses. In this study, we attempted to determine whether the anti-CD3 single-chain Fv/IL-18 fusion DNA (pAnti-CD3sFv/IL-18) could stimulate resistance to Mycobacterium avium infection in genetically susceptible BALB/c mice. Immunization with pAnti-CD3sFv/IL-18 DNA during intranasal infection with M. avium resulted in a significant reduction in the bacterial load in the lung throughout the entire 10-week observation period. Immunization with pAnti-CD3sFv/IL-18 DNA induced and maintained significantly higher levels of cytotoxic activity and nitric oxide production by the lung cells, as compared with the injection of pAnti-CD3sFv DNA, or of a simple mixture of pAnti-CD3sFv DNA and pIL-18 DNA. Furthermore, the lung cells of the mice injected with pAnti-CD3sFv/IL-18 DNA exhibited persistent production of IFN-γ. Treatment with anti-IFN-γ antibody resulted in an abrogation of anti-mycobacterial effects in mice injected with pAnti-CD3sFv/IL-18 DNA. These results indicate that vaccination with the pAnti-CD3sFv/IL-18 fusion DNA induces significant resistance to M. avium infection, via the production of IFN-γ in CD4+ T cells.

KW - CD3 T cell

KW - Fusion DNA

KW - IL-18

KW - Immunity

KW - Mycobacterium avium

UR - http://www.scopus.com/inward/record.url?scp=33645069658&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2005.12.065

DO - 10.1016/j.vaccine.2005.12.065

M3 - Article

C2 - 16481075

AN - SCOPUS:33645069658

SN - 0264-410X

VL - 24

SP - 3365

EP - 3373

JO - Vaccine

JF - Vaccine

IS - 16

ER -

Anti-CD3 single-chain Fv/interleukin-18 fusion DNA induces anti-mycobacterial resistance via efficient interferon-γ production in BALB/c mice infected with Mycobacterium avium

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this