Anti-complementary properties of polysaccharides isolated from fruit bodies of mushroom Pleurotus ostreatus

Mee Hyang Kweon, Hyo Jang, Wang Jin Lim, Hyo Ihl Chang, Chan Wha Kim, Han Chul Yang, Han Joon Hwang, Ha Chin Sung

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18 Citations (Scopus)


A high molecular-weight water-soluble fraction (PO) obtained by the ethanol precipitation of 0.1 N NaOH extracts of the mushroom Pleurotus ostreatus showed 82% anti-complementary activity for complement consumption hemolysis. The PO consisted of 42% carbohydrate (w/w), 50% protein (w/w), and 3% uronic acid (w/w). Fifty-eight percent of the anti-complementary activity decreased by periodate oxidation and 22% by protease digestion, suggesting that the sugar and protein moieties are essential for this activity. Two polysaccharide fractions, PO-IIIa-1 and PO-IIIa-2, with anti-complementary activity were isolated from the PO using DEAE-Sepharose FF followed by Sephadex G75 and Sepharose CL-6B gel permeation chromatographies. The PO- IIIa-2 was found by HPLC to be nearly homogeneous, with the molecular mass of 531 kDa, and showed 96% ITCH50 (inhibition against the total complement hemolysis of deionized water as the control) at a concentration of 1 mg/ml. This fraction contained galactose, mannose, fucose, and glucose with molar ratios of 1.75:1:0.65 and 0.59, respectively. The majority of galactose and mannose units in the PO-IIIa-2 were composed of TGalpl→, →6Galpl→, →2,6Galpl→, and →Manpl→. The PO-IIIa-1 (molecular mass of 2000 kDa), exhibiting higher activity than the PO-IIIa-2, was further purified into two fractions, unbound proteoglycan (PO-IIIa-1A) and bound glucomannan (PO-IIIa- 1B), by affinity chromatography using ConA-Sepharose CL-4B. The anti- complementary activity of each affinity purified fraction decreased as compared to that of the native PO-IIIa-1 fraction, indicating that the formation of complex between both polysaccharide fractions was necessary for full anticomplementary activity.

Original languageEnglish
Pages (from-to)450-456
Number of pages7
JournalJournal of microbiology and biotechnology
Issue number4
Publication statusPublished - 1999 Aug


  • Aggregation
  • Anti-complementary
  • Pleurotus ostreatus
  • Polysaccharides

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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