Anti-inflammatory action of ethanolic extract of Ramulus mori on the BLT2-linked cascade

Geun Soo Park, Jeong Keun Kim, Jae Hong Kim

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Mulberry tree twigs (Ramulus mori) contain large amounts of oxyresveratrols and have traditionally been used as herbal medicines because of their anti-inflammatory properties. However, the signaling mechanism by which R. mori exerts its anti-inflammatory action remains to be elucidated. In this study, we observed that R. mori ethanol extracts (RME) exerted an inhibitory effect on the lipopolysaccharide (LPS)-induced production of the pro-inflammatory cytokine interleukin-6 (IL-6) in Raw264.7 macrophage cells. Additionally, RME inhibited IL-6 production by blocking the leukotriene B4 receptor-2 (BLT2)-dependent-NADPH oxidase 1 (NOX1)-reactive oxygen species (ROS) cascade, leading to anti-inflammatory activity. Finally, RME suppressed the production of the BLT2 ligands LTB4 and 12(S)-HETE by inhibiting the p38 kinase-cytosolic phospholipase A2-5-/12-lipoxygenase cascade in LPS-stimulated Raw264.7 cells. Overall, our results suggest that RME inhibits the 'BLT2 ligand-BLT2'-linked autocrine inflammatory axis, and that this BLT2-linked cascade is one of the targets of the anti-inflammatory action of R. mori.

Original languageEnglish
Pages (from-to)232-237
Number of pages6
JournalBMB reports
Issue number4
Publication statusPublished - 2016 Apr 1

Bibliographical note

Publisher Copyright:
© 2016 by the The Korean Society for Biochemistry and Molecular Biology.


  • BLT2
  • IL-6
  • ROS
  • Ramulus mori
  • Raw264.7 cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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