Anti-inflammatory withanolides from the aerial parts of Physalis minima

Wenhui Liu; Han Zhang; Hongxu Wan; Jiantong Hou; Dongho Lee; Jing Xu; Yuanqiang Guo

doi:10.1016/j.phytochem.2022.113301

Anti-inflammatory withanolides from the aerial parts of Physalis minima

Wenhui Liu, Han Zhang, Hongxu Wan, Jiantong Hou, Dongho Lee, Jing Xu, Yuanqiang Guo

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Eight undescribed and two known withanolides were obtained from the aerial parts of Physalis minima. The structures of these compounds were defined by spectroscopic methods including 1D and 2D NMR, HRESIMS, and electronic circular dichroism (ECD) data analysis. Physminin E was elucidated to be a rare 13,14-seco-withanolide. Inhibitory effects of these compounds on nitric oxide (NO) production were evaluated by using LPS-activated RAW264.7 macrophages, and physminin C was shown to be the most active with an IC₅₀ value of 3.5 μM. The further mechanistic analysis of NO inhibition was performed by molecular docking and Western blotting.

Original language	English
Article number	113301
Journal	Phytochemistry
Volume	202
DOIs	https://doi.org/10.1016/j.phytochem.2022.113301
Publication status	Published - 2022 Oct

Bibliographical note

Funding Information:
This research was supported financially by the National Key Research and Development Program of China (No. 2018YFA0507204 ), and the National Natural Science Foundation of China (Nos. 22077067 , 22177054 , and U1801288 ).

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

Molecular docking
Nitric oxide inhibitory activities
Physalis minima
Solanaceae
Withanolides

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

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10.1016/j.phytochem.2022.113301

Cite this

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title = "Anti-inflammatory withanolides from the aerial parts of Physalis minima",

abstract = "Eight undescribed and two known withanolides were obtained from the aerial parts of Physalis minima. The structures of these compounds were defined by spectroscopic methods including 1D and 2D NMR, HRESIMS, and electronic circular dichroism (ECD) data analysis. Physminin E was elucidated to be a rare 13,14-seco-withanolide. Inhibitory effects of these compounds on nitric oxide (NO) production were evaluated by using LPS-activated RAW264.7 macrophages, and physminin C was shown to be the most active with an IC50 value of 3.5 μM. The further mechanistic analysis of NO inhibition was performed by molecular docking and Western blotting.",

keywords = "Molecular docking, Nitric oxide inhibitory activities, Physalis minima, Solanaceae, Withanolides",

author = "Wenhui Liu and Han Zhang and Hongxu Wan and Jiantong Hou and Dongho Lee and Jing Xu and Yuanqiang Guo",

note = "Funding Information: This research was supported financially by the National Key Research and Development Program of China (No. 2018YFA0507204 ), and the National Natural Science Foundation of China (Nos. 22077067 , 22177054 , and U1801288 ). Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

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doi = "10.1016/j.phytochem.2022.113301",

language = "English",

volume = "202",

journal = "Phytochemistry",

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T1 - Anti-inflammatory withanolides from the aerial parts of Physalis minima

AU - Liu, Wenhui

AU - Zhang, Han

AU - Wan, Hongxu

AU - Hou, Jiantong

AU - Lee, Dongho

AU - Xu, Jing

AU - Guo, Yuanqiang

N1 - Funding Information: This research was supported financially by the National Key Research and Development Program of China (No. 2018YFA0507204 ), and the National Natural Science Foundation of China (Nos. 22077067 , 22177054 , and U1801288 ). Publisher Copyright: © 2022 Elsevier Ltd

PY - 2022/10

Y1 - 2022/10

N2 - Eight undescribed and two known withanolides were obtained from the aerial parts of Physalis minima. The structures of these compounds were defined by spectroscopic methods including 1D and 2D NMR, HRESIMS, and electronic circular dichroism (ECD) data analysis. Physminin E was elucidated to be a rare 13,14-seco-withanolide. Inhibitory effects of these compounds on nitric oxide (NO) production were evaluated by using LPS-activated RAW264.7 macrophages, and physminin C was shown to be the most active with an IC50 value of 3.5 μM. The further mechanistic analysis of NO inhibition was performed by molecular docking and Western blotting.

AB - Eight undescribed and two known withanolides were obtained from the aerial parts of Physalis minima. The structures of these compounds were defined by spectroscopic methods including 1D and 2D NMR, HRESIMS, and electronic circular dichroism (ECD) data analysis. Physminin E was elucidated to be a rare 13,14-seco-withanolide. Inhibitory effects of these compounds on nitric oxide (NO) production were evaluated by using LPS-activated RAW264.7 macrophages, and physminin C was shown to be the most active with an IC50 value of 3.5 μM. The further mechanistic analysis of NO inhibition was performed by molecular docking and Western blotting.

KW - Molecular docking

KW - Nitric oxide inhibitory activities

KW - Physalis minima

KW - Solanaceae

KW - Withanolides

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DO - 10.1016/j.phytochem.2022.113301

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SN - 0031-9422

VL - 202

JO - Phytochemistry

JF - Phytochemistry

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Anti-inflammatory withanolides from the aerial parts of Physalis minima

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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