Anti-ischemic and anti-inflammatory activity of (S)-cis-verbenol

In Young Choi, Ji H. Lim, Sunyoung Hwang, Jae Chul Lee, Geum Sil Cho, Won Ki Kim

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

(S)-cis-verbenol, a natural metabolite from (-)-alpha-pinene of host pine tree, has been suggested to have anti-ischemic activity. However, the exact mechanism for the anti-ischemic activity of (S)-cis-verbenol remains unclear yet. In the present study, (S)-cis-verbenol reduced cerebral ischemic injury caused by 1.5-h middle cerebral artery occlusion followed by 24-h reperfusion. Furthermore, (S)-cis-verbenol signifi cantly prevented neuronal cell death caused by oxygen-glucose deprivation (OGD, 1 h) and subsequent re-oxygenation (5 h). While (S)-cis-verbenol did not inhibit the NMDA-stimulated calcium infl ux, it reduced the intracellular level of reactive oxygen species (ROS) elevated by OGD/re-oxygenation. ORAC assay indicated that (S)-cis-verbenol potently eliminated peroxyl radicals. In DPPH and DHR123 fl uorescence assays, however, (S)-cis-verbenol did not show a direct ROS scavenging effect. Furthermore, (S)-cis-verbenol reduced the expression levels of pro-infl ammatory cytokines in ischemic brain and immunostimulated glial cells. The present results indicate that (S)-cis-verbenol may be a useful therapeutic agent due to its anti-oxidative and anti-infl ammatory activities.

Original languageEnglish
Pages (from-to)541-551
Number of pages11
JournalFree Radical Research
Volume44
Issue number5
DOIs
Publication statusPublished - 2010
Externally publishedYes

Bibliographical note

Funding Information:
Declaration of interest: This study was supported by a grant (#2009K001250) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology and a grant from the Brain Korea 21 Project (to Dr. I. Y. Choi), the Republic of Korea. The authors report no conflicts of interest.The authors alone are responsible for the content and writing of the paper.

Keywords

  • (S)-cis-verbenol
  • Infl ammation
  • Ischemia
  • OGD/re-oxygenation
  • Peroxyl radicals
  • ROS

ASJC Scopus subject areas

  • Biochemistry

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