Anticancer activity of lactoferrin isolated from caprine colostrum on human cancer cell lines

Younghoon Kim; Mae Jin Kim; Kyoung Sik Han; Jee Young Imm; Sejong Oh; Sae Hun Kim

doi:10.1111/j.1471-0307.2009.00466.x

Anticancer activity of lactoferrin isolated from caprine colostrum on human cancer cell lines

Younghoon Kim, Mae Jin Kim, Kyoung Sik Han, Jee Young Imm, Sejong Oh, Sae Hun Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

In the present study, lactoferrin was purified from caprine colostrum and its anticancer effects were investigated on various cancer cell lines, including lung, colon, cervix, stomach and breast cancer cells. The caprine lactoferrin (CLF) was purified by ultrafiltration and sequential chromatography using a CM-Sephadex C-50-120 ion-exchange column and affinity chromatography on Hi-Trap^TM Heparin HP. The purified CLF exhibited antiproliferative effects on the five tested cancer cell lines in a dose-dependent manner, with 20-30% survival as compared to the control. Interestingly, the most intense inhibition of cell growth was observed on the ZR-75-1 breast cancer cells (IC₅₀ = 27.5 μg/mL). Our findings suggest, for the first time, that purified CLF from caprine colostrum may constitute a novel anticancer agent for the food industry.

Original language	English
Pages (from-to)	277-281
Number of pages	5
Journal	International Journal of Dairy Technology
Volume	62
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-0307.2009.00466.x
Publication status	Published - 2009 May

Keywords

Anticancer activity
Caprine lactoferrin
Chromatography
Human cancer cell lines
Purification

ASJC Scopus subject areas

Food Science
Bioengineering
Process Chemistry and Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/j.1471-0307.2009.00466.x

Cite this

@article{6bdecc17342640658c2b811513135023,

title = "Anticancer activity of lactoferrin isolated from caprine colostrum on human cancer cell lines",

abstract = "In the present study, lactoferrin was purified from caprine colostrum and its anticancer effects were investigated on various cancer cell lines, including lung, colon, cervix, stomach and breast cancer cells. The caprine lactoferrin (CLF) was purified by ultrafiltration and sequential chromatography using a CM-Sephadex C-50-120 ion-exchange column and affinity chromatography on Hi-TrapTM Heparin HP. The purified CLF exhibited antiproliferative effects on the five tested cancer cell lines in a dose-dependent manner, with 20-30% survival as compared to the control. Interestingly, the most intense inhibition of cell growth was observed on the ZR-75-1 breast cancer cells (IC50 = 27.5 μg/mL). Our findings suggest, for the first time, that purified CLF from caprine colostrum may constitute a novel anticancer agent for the food industry.",

keywords = "Anticancer activity, Caprine lactoferrin, Chromatography, Human cancer cell lines, Purification",

author = "Younghoon Kim and Kim, {Mae Jin} and Han, {Kyoung Sik} and Imm, {Jee Young} and Sejong Oh and Kim, {Sae Hun}",

year = "2009",

month = may,

doi = "10.1111/j.1471-0307.2009.00466.x",

language = "English",

volume = "62",

pages = "277--281",

journal = "International Journal of Dairy Technology",

issn = "1364-727X",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Anticancer activity of lactoferrin isolated from caprine colostrum on human cancer cell lines

AU - Kim, Younghoon

AU - Kim, Mae Jin

AU - Han, Kyoung Sik

AU - Imm, Jee Young

AU - Oh, Sejong

AU - Kim, Sae Hun

PY - 2009/5

Y1 - 2009/5

N2 - In the present study, lactoferrin was purified from caprine colostrum and its anticancer effects were investigated on various cancer cell lines, including lung, colon, cervix, stomach and breast cancer cells. The caprine lactoferrin (CLF) was purified by ultrafiltration and sequential chromatography using a CM-Sephadex C-50-120 ion-exchange column and affinity chromatography on Hi-TrapTM Heparin HP. The purified CLF exhibited antiproliferative effects on the five tested cancer cell lines in a dose-dependent manner, with 20-30% survival as compared to the control. Interestingly, the most intense inhibition of cell growth was observed on the ZR-75-1 breast cancer cells (IC50 = 27.5 μg/mL). Our findings suggest, for the first time, that purified CLF from caprine colostrum may constitute a novel anticancer agent for the food industry.

AB - In the present study, lactoferrin was purified from caprine colostrum and its anticancer effects were investigated on various cancer cell lines, including lung, colon, cervix, stomach and breast cancer cells. The caprine lactoferrin (CLF) was purified by ultrafiltration and sequential chromatography using a CM-Sephadex C-50-120 ion-exchange column and affinity chromatography on Hi-TrapTM Heparin HP. The purified CLF exhibited antiproliferative effects on the five tested cancer cell lines in a dose-dependent manner, with 20-30% survival as compared to the control. Interestingly, the most intense inhibition of cell growth was observed on the ZR-75-1 breast cancer cells (IC50 = 27.5 μg/mL). Our findings suggest, for the first time, that purified CLF from caprine colostrum may constitute a novel anticancer agent for the food industry.

KW - Anticancer activity

KW - Caprine lactoferrin

KW - Chromatography

KW - Human cancer cell lines

KW - Purification

UR - http://www.scopus.com/inward/record.url?scp=66149103175&partnerID=8YFLogxK

U2 - 10.1111/j.1471-0307.2009.00466.x

DO - 10.1111/j.1471-0307.2009.00466.x

M3 - Article

AN - SCOPUS:66149103175

SN - 1364-727X

VL - 62

SP - 277

EP - 281

JO - International Journal of Dairy Technology

JF - International Journal of Dairy Technology

IS - 2

ER -

Anticancer activity of lactoferrin isolated from caprine colostrum on human cancer cell lines

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this