This paper presents highly effective antimicrobial surfaces employing a 2D structured nanobiocatalyst composed of graphene oxide (GO) and glucose oxidase (GOD). Enzyme molecules are immobilized onto extra-large GO pieces with a plane dimension of approximately 100 μm via an enzyme adsorption, precipitation, and crosslinking (EAPC) approach. This enables the effective wrapping of extra-large GO pieces by a matrix of crosslinked enzyme aggregates, which improves the enzyme loading. Consequently, the measured GOD activities of the EAPC sample via 50% (w/v) ammonium sulfate precipitation are 4,940 and 3,820 times higher than those of the control samples, i.e, the enzyme adsorption (EA) and enzyme adsorption/crosslinking (EAC) samples, respectively. The preservation of the planar GO geometry with an extra-large surface also allows the effective binding of EAPC onto a commercial membrane filter via a polydopamine coating, thus yielding a biocatalytic EAPC membrane. Compared to the commercial membrane with no bound EAPC, the in situ generation of H2O2 via the EAPC-catalyzed oxidation of glucose on the membrane surface demonstrated enhanced filterability against a mixed bacterial population of activated sludge obtained from a municipal sewage plant as well as two model bacteria: gram-negative Pseudomonas aeruginosa and gram-positive Staphylococcus aureus. The bacterial decontamination of the EAPC-bound membrane surface can also be activated on demand by simply adding glucose to the bulk solution. This newly proposed mechanism of antifouling surfaces employing a localized nanobiocatalytic conversion of nontoxic glucose to bactericidal H2O2 can provide insights for biofouling control via a highly effective and environment-friendly approach.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government Ministry of Science and ICT (MSIT) of the Republic of Korea. (Nos. NRF-2020R1A2C3009649 and NRF-2014K1A1A2043032).
This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government Ministry of Science and ICT (MSIT) of the Republic of Korea . (Nos. NRF-2020R1A2C3009649 and NRF-2014K1A1A2043032 ).
© 2021 Elsevier B.V.
- Antimicrobial surface
- Glucose oxidase
- Graphene oxide
- In situ biocide generation
- Membrane antifouling
- Planar nanobiocatalyst
ASJC Scopus subject areas
- Environmental Chemistry
- Chemical Engineering(all)
- Industrial and Manufacturing Engineering