Antifouling membranes employing a 2D planar nanobiocatalyst of crosslinked glucose oxidase aggregates wrapping extra-large graphene oxide

Testaverde S. Kim, Jahyun Nam, Dae Woo Kim, Hee Tae Jung, Kyung Min Yeon, Jungbae Kim

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

This paper presents highly effective antimicrobial surfaces employing a 2D structured nanobiocatalyst composed of graphene oxide (GO) and glucose oxidase (GOD). Enzyme molecules are immobilized onto extra-large GO pieces with a plane dimension of approximately 100 μm via an enzyme adsorption, precipitation, and crosslinking (EAPC) approach. This enables the effective wrapping of extra-large GO pieces by a matrix of crosslinked enzyme aggregates, which improves the enzyme loading. Consequently, the measured GOD activities of the EAPC sample via 50% (w/v) ammonium sulfate precipitation are 4,940 and 3,820 times higher than those of the control samples, i.e, the enzyme adsorption (EA) and enzyme adsorption/crosslinking (EAC) samples, respectively. The preservation of the planar GO geometry with an extra-large surface also allows the effective binding of EAPC onto a commercial membrane filter via a polydopamine coating, thus yielding a biocatalytic EAPC membrane. Compared to the commercial membrane with no bound EAPC, the in situ generation of H2O2 via the EAPC-catalyzed oxidation of glucose on the membrane surface demonstrated enhanced filterability against a mixed bacterial population of activated sludge obtained from a municipal sewage plant as well as two model bacteria: gram-negative Pseudomonas aeruginosa and gram-positive Staphylococcus aureus. The bacterial decontamination of the EAPC-bound membrane surface can also be activated on demand by simply adding glucose to the bulk solution. This newly proposed mechanism of antifouling surfaces employing a localized nanobiocatalytic conversion of nontoxic glucose to bactericidal H2O2 can provide insights for biofouling control via a highly effective and environment-friendly approach.

Original languageEnglish
Article number130343
JournalChemical Engineering Journal
Volume424
DOIs
Publication statusPublished - 2021 Nov 15

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government Ministry of Science and ICT (MSIT) of the Republic of Korea. (Nos. NRF-2020R1A2C3009649 and NRF-2014K1A1A2043032).

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government Ministry of Science and ICT (MSIT) of the Republic of Korea . (Nos. NRF-2020R1A2C3009649 and NRF-2014K1A1A2043032 ).

Publisher Copyright:
© 2021 Elsevier B.V.

Keywords

  • Antimicrobial surface
  • Glucose oxidase
  • Graphene oxide
  • In situ biocide generation
  • Membrane antifouling
  • Planar nanobiocatalyst

ASJC Scopus subject areas

  • General Chemistry
  • Environmental Chemistry
  • General Chemical Engineering
  • Industrial and Manufacturing Engineering

Fingerprint

Dive into the research topics of 'Antifouling membranes employing a 2D planar nanobiocatalyst of crosslinked glucose oxidase aggregates wrapping extra-large graphene oxide'. Together they form a unique fingerprint.

Cite this