Antigrowth effects of Kaempferia parviflora extract enriched in anthocyanidins on human ovarian cancer cells through Ca2+-ROS overload and mitochondrial dysfunction

Myung Hyun Kim, Sunwoo Park, Gwonhwa Song, Whasun Lim, Young Sil Han

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Background: Ovarian cancer is hard to diagnose and its survival rates dramatically decrease according to the stage progression. Anthocyanidins derived from Kaempferia parviflora has been studied for anti-inflammatory, antioxidant, and anticancer effects in various disease both in vitro and in vivo. However, no studies have evaluated the molecular mechanism of Kaempferia parviflora anthocyanidin fractions in ovarian cancer cells. Objectives: To study the effects of Kaempferia parviflora anthocyanidins on the progression of ovarian cancer, we extracted anthocyanidins from Kaempferia parviflora and analyzed it using HPLC. Then, we determined the change of cell characteristics by treatment of anthocyanidins derived from Kaempferia parviflora. Results: Based on HPLC analysis, the most abundant anthocyanidins of Kaempferia parviflora was peonidin, followed by cyanidin, delphinidin, and pelargonidin. We selected high-grade serous ovarian cancer and clear cell cancer cell lines to investigate the anticancer effects of anthocyanidin extracts. Treatment with anthocyanidin extracts decreased ES-2 and OV-90 cell proliferation and increased late apoptosis with cell cycle arrest at sub G0/G1 phase. In addition, anthocyanidin extracts hampered the mitochondrial membrane permeability in both cell lines. Moreover, the cytosolic calcium accumulation was detected in ES-2 cells, and the overproduction of reactive oxygen species was estimated in OV-90 cells, respectively. Conclusion: Collectively, these results showed the anticancer effects of Kaempferia parviflora anthocyanidin extracts against the progression of ovarian cancer.

Original languageEnglish
Pages (from-to)383-391
Number of pages9
JournalMolecular and Cellular Toxicology
Issue number3
Publication statusPublished - 2022 Jul

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (grant number: 2021R1C1C1009807 and 2021R1A2C2005841) and funded by Ministry of Education (grant number: 2020R1I1A1A01067648). This study also was supported by "Cooperative Research Program for Agriculture Science & Technology Development (Project No.PJ01608201)" Rural Development Administration, Republic of Korea.

Publisher Copyright:
© 2021, The Author(s) under exclusive licence to The Korean Society of Toxicogenomics and Toxicoproteomics.


  • Anthocyanidin extracts
  • Apoptosis
  • Kaempferia parviflora
  • Mitochondrial dysfunction
  • Proliferation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis


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