Antioxidative role of selenoprotein W in oxidant-induced mouse embryonic neuronal cell death

Youn Wook Chung; Daewon Jeong; Ok Jeong Noh; Yong Hwan Park; Soo Im Kang; Min Goo Lee; Tae Hoon Lee; Moon Bin Yim; Ick Young Kim

doi:10.1007/s10059-009-0074-3

Antioxidative role of selenoprotein W in oxidant-induced mouse embryonic neuronal cell death

Youn Wook Chung, Daewon Jeong, Ok Jeong Noh, Yong Hwan Park, Soo Im Kang, Min Goo Lee, Tae Hoon Lee, Moon Bin Yim, Ick Young Kim

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats, and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1 and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced by treatment of H₂O₂ than control cells. TUNEL assays revealed that H₂O₂-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal development.

Original language	English
Pages (from-to)	609-613
Number of pages	5
Journal	Molecules and cells
Volume	27
Issue number	5
DOIs	https://doi.org/10.1007/s10059-009-0074-3
Publication status	Published - 2009

Keywords

Antioxidant
Neuronal cells
Oxidative stress
Selenium
Seleno protein W

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Access to Document

10.1007/s10059-009-0074-3

Cite this

@article{cf9b80f0e0e049abba41912019c15164,

title = "Antioxidative role of selenoprotein W in oxidant-induced mouse embryonic neuronal cell death",

abstract = "It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats, and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1 and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced by treatment of H2O2 than control cells. TUNEL assays revealed that H2O2-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal development.",

keywords = "Antioxidant, Neuronal cells, Oxidative stress, Selenium, Seleno protein W",

author = "Chung, {Youn Wook} and Daewon Jeong and Noh, {Ok Jeong} and Park, {Yong Hwan} and Kang, {Soo Im} and Lee, {Min Goo} and Lee, {Tae Hoon} and Yim, {Moon Bin} and Kim, {Ick Young}",

note = "Funding Information: We thank Dr. P.B. Chock (Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, USA), Dr. W. Sun and Dr. H. Kim (Department of Anatomy, College of Medicine, Korea University, Seoul, Korea), and Dr. J.H. Baik (School of Life Sciences and Biotechnology, Korea University, Seoul, Korea) for helpful discussions. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (Ministry of Education and Human Resources Development) (KRF-2005-070-C00086) and a Korea University Grant.",

year = "2009",

doi = "10.1007/s10059-009-0074-3",

language = "English",

volume = "27",

pages = "609--613",

journal = "Molecules and Cells",

issn = "1016-8478",

publisher = "Korean Society for Molecular and Cellular Biology",

number = "5",

}

TY - JOUR

T1 - Antioxidative role of selenoprotein W in oxidant-induced mouse embryonic neuronal cell death

AU - Chung, Youn Wook

AU - Jeong, Daewon

AU - Noh, Ok Jeong

AU - Park, Yong Hwan

AU - Kang, Soo Im

AU - Lee, Min Goo

AU - Lee, Tae Hoon

AU - Yim, Moon Bin

AU - Kim, Ick Young

N1 - Funding Information: We thank Dr. P.B. Chock (Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, USA), Dr. W. Sun and Dr. H. Kim (Department of Anatomy, College of Medicine, Korea University, Seoul, Korea), and Dr. J.H. Baik (School of Life Sciences and Biotechnology, Korea University, Seoul, Korea) for helpful discussions. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (Ministry of Education and Human Resources Development) (KRF-2005-070-C00086) and a Korea University Grant.

PY - 2009

Y1 - 2009

N2 - It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats, and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1 and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced by treatment of H2O2 than control cells. TUNEL assays revealed that H2O2-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal development.

AB - It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats, and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1 and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced by treatment of H2O2 than control cells. TUNEL assays revealed that H2O2-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal development.

KW - Antioxidant

KW - Neuronal cells

KW - Oxidative stress

KW - Selenium

KW - Seleno protein W

UR - http://www.scopus.com/inward/record.url?scp=66649090050&partnerID=8YFLogxK

U2 - 10.1007/s10059-009-0074-3

DO - 10.1007/s10059-009-0074-3

M3 - Article

C2 - 19466610

AN - SCOPUS:66649090050

SN - 1016-8478

VL - 27

SP - 609

EP - 613

JO - Molecules and Cells

JF - Molecules and Cells

IS - 5

ER -

Antioxidative role of selenoprotein W in oxidant-induced mouse embryonic neuronal cell death

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this