It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats, and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1 and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced by treatment of H2O2 than control cells. TUNEL assays revealed that H2O2-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal development.
Bibliographical noteFunding Information:
We thank Dr. P.B. Chock (Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, USA), Dr. W. Sun and Dr. H. Kim (Department of Anatomy, College of Medicine, Korea University, Seoul, Korea), and Dr. J.H. Baik (School of Life Sciences and Biotechnology, Korea University, Seoul, Korea) for helpful discussions. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (Ministry of Education and Human Resources Development) (KRF-2005-070-C00086) and a Korea University Grant.
- Neuronal cells
- Oxidative stress
- Seleno protein W
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology