Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157:H7

L. E.E. Kang-Mu; K. I.M. Wan-Seok; L. I.M. Jeesun; N. A.M. Sunyoung; M. I.N. Youn; N. A.M. Seong-Won; K. I.M. Younghoon; K. I.M. Sae-Hun; Woojun Park; Sungsu Park

doi:10.4315/0362-028x-72.2.325

Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157:H7

L. E.E. Kang-Mu, K. I.M. Wan-Seok, L. I.M. Jeesun, N. A.M. Sunyoung, M. I.N. Youn, N. A.M. Seong-Won, K. I.M. Younghoon, K. I.M. Sae-Hun, Woojun Park, Sungsu Park

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67 Citations (Scopus)

Abstract

The inhibitory effects of green tea polyphenol epigallocatechin gallate (EGCG) on virulence phenotypes and gene expression regulated by quorum sensing (QS) in Escherichia coli O157:H7 were demonstrated at concentrations of 1 to 100 μg/ml, which are lower than the MIC (539 ± 22 μ-g/ml). At 25 μg/ml, the growth rate was not affected, but autoinducer 2 concentration, biofilm formation, and swarm motility decreased to 13.2, 11.8, and 50%, respectively. Survival at 5 days of nematodes (Caenorhabditis elegans) that were fed the pathogen without and with EGCG were 47.1 and 76%, respectively. Real-time PCR data indicated decreased transcriptional level in many quorum sensing-regulated virulence genes at 25 μg/ml. Our results suggest that EGCG at concentrations below its MIC has significant antipathogenic effects against E. coli O157:H7.

Original language	English
Pages (from-to)	325-331
Number of pages	7
Journal	Journal of Food Protection
Volume	72
Issue number	2
DOIs	https://doi.org/10.4315/0362-028x-72.2.325
Publication status	Published - 2009 Feb

ASJC Scopus subject areas

Food Science
Microbiology

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10.4315/0362-028x-72.2.325

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Kang-Mu, L. E. E., Wan-Seok, K. I. M., Jeesun, L. I. M., Sunyoung, N. A. M., Youn, M. I. N., Seong-Won, N. A. M., Younghoon, K. I. M., Sae-Hun, K. I. M., Park, W., & Park, S. (2009). Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157:H7. Journal of Food Protection, 72(2), 325-331. https://doi.org/10.4315/0362-028x-72.2.325

Kang-Mu, LEE, Wan-Seok, KIM, Jeesun, LIM, Sunyoung, NAM, Youn, MIN, Seong-Won, NAM, Younghoon, KIM, Sae-Hun, KIM , Park, W & Park, S 2009, 'Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157:H7', Journal of Food Protection, vol. 72, no. 2, pp. 325-331. https://doi.org/10.4315/0362-028x-72.2.325

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title = "Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157:H7",

abstract = "The inhibitory effects of green tea polyphenol epigallocatechin gallate (EGCG) on virulence phenotypes and gene expression regulated by quorum sensing (QS) in Escherichia coli O157:H7 were demonstrated at concentrations of 1 to 100 μg/ml, which are lower than the MIC (539 ± 22 μ-g/ml). At 25 μg/ml, the growth rate was not affected, but autoinducer 2 concentration, biofilm formation, and swarm motility decreased to 13.2, 11.8, and 50%, respectively. Survival at 5 days of nematodes (Caenorhabditis elegans) that were fed the pathogen without and with EGCG were 47.1 and 76%, respectively. Real-time PCR data indicated decreased transcriptional level in many quorum sensing-regulated virulence genes at 25 μg/ml. Our results suggest that EGCG at concentrations below its MIC has significant antipathogenic effects against E. coli O157:H7.",

author = "Kang-Mu, {L. E.E.} and Wan-Seok, {K. I.M.} and Jeesun, {L. I.M.} and Sunyoung, {N. A.M.} and Youn, {M. I.N.} and Seong-Won, {N. A.M.} and Younghoon, {K. I.M.} and Sae-Hun, {K. I.M.} and Woojun Park and Sungsu Park",

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doi = "10.4315/0362-028x-72.2.325",

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AU - Kang-Mu, L. E.E.

AU - Wan-Seok, K. I.M.

AU - Jeesun, L. I.M.

AU - Sunyoung, N. A.M.

AU - Youn, M. I.N.

AU - Seong-Won, N. A.M.

AU - Younghoon, K. I.M.

AU - Sae-Hun, K. I.M.

AU - Park, Woojun

AU - Park, Sungsu

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N2 - The inhibitory effects of green tea polyphenol epigallocatechin gallate (EGCG) on virulence phenotypes and gene expression regulated by quorum sensing (QS) in Escherichia coli O157:H7 were demonstrated at concentrations of 1 to 100 μg/ml, which are lower than the MIC (539 ± 22 μ-g/ml). At 25 μg/ml, the growth rate was not affected, but autoinducer 2 concentration, biofilm formation, and swarm motility decreased to 13.2, 11.8, and 50%, respectively. Survival at 5 days of nematodes (Caenorhabditis elegans) that were fed the pathogen without and with EGCG were 47.1 and 76%, respectively. Real-time PCR data indicated decreased transcriptional level in many quorum sensing-regulated virulence genes at 25 μg/ml. Our results suggest that EGCG at concentrations below its MIC has significant antipathogenic effects against E. coli O157:H7.

AB - The inhibitory effects of green tea polyphenol epigallocatechin gallate (EGCG) on virulence phenotypes and gene expression regulated by quorum sensing (QS) in Escherichia coli O157:H7 were demonstrated at concentrations of 1 to 100 μg/ml, which are lower than the MIC (539 ± 22 μ-g/ml). At 25 μg/ml, the growth rate was not affected, but autoinducer 2 concentration, biofilm formation, and swarm motility decreased to 13.2, 11.8, and 50%, respectively. Survival at 5 days of nematodes (Caenorhabditis elegans) that were fed the pathogen without and with EGCG were 47.1 and 76%, respectively. Real-time PCR data indicated decreased transcriptional level in many quorum sensing-regulated virulence genes at 25 μg/ml. Our results suggest that EGCG at concentrations below its MIC has significant antipathogenic effects against E. coli O157:H7.

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Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic escherichia coli O157:H7

Abstract

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