Antiviral activity of angelicin against gammaherpesviruses

Hye Jeong Cho; Seon Gyeong Jeong; Ji Eun Park; Jin Ah Han; Hye Ri Kang; Dongho Lee; Moon Jung Song

doi:10.1016/j.antiviral.2013.07.009

Antiviral activity of angelicin against gammaherpesviruses

Hye Jeong Cho, Seon Gyeong Jeong, Ji Eun Park, Jin Ah Han, Hye Ri Kang, Dongho Lee, Moon Jung Song

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Human gammaherpesviruses including Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are important pathogens as they persist in the host and cause various malignancies. However, few antiviral drugs are available to efficiently control gammaherpesvirus replication. Here we identified the antiviral activity of angelicin against murine gammaherpesvirus 68 (MHV-68), genetically and biologically related to human gammaherpesviruses. Angelicin, a furocoumarin naturally occurring tricyclic aromatic compound, efficiently inhibited lytic replication of MHV-68 in a dose-dependent manner following the virus entry. The IC₅₀ of angelicin antiviral activity was estimated to be 28.95 μM, while the CC₅₀ of angelicin was higher than 2600 μM. Furthermore, incubation with angelicin efficiently inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced lytic replication of human gammaherpresviruses in both EBV- and KSHV-infected cells. Taken together, these results suggest that MHV-68 can be a useful tool to screen novel antiviral agents against human gammaherepsviruses and that angelicin may provide a lead structure for the development of antiviral drug against gammaherpesviruses.

Original language	English
Pages (from-to)	75-83
Number of pages	9
Journal	Antiviral Research
Volume	100
Issue number	1
DOIs	https://doi.org/10.1016/j.antiviral.2013.07.009
Publication status	Published - 2013

Bibliographical note

Funding Information:
This study was supported by a Korea University Grant, a Grant of the Korea Healthcare technology R&D Project, Ministry of Health, Welfare & Family Affairs, Republic of Korea (A091021), the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MEST) (2008-2005321) and Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries (2011–2013), Republic of Korea to M.J.S.

Keywords

Angelicin
Antiviral activity
EBV
Furocoumarins
KSHV
MHV-68

ASJC Scopus subject areas

Pharmacology
Virology

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10.1016/j.antiviral.2013.07.009

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title = "Antiviral activity of angelicin against gammaherpesviruses",

abstract = "Human gammaherpesviruses including Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are important pathogens as they persist in the host and cause various malignancies. However, few antiviral drugs are available to efficiently control gammaherpesvirus replication. Here we identified the antiviral activity of angelicin against murine gammaherpesvirus 68 (MHV-68), genetically and biologically related to human gammaherpesviruses. Angelicin, a furocoumarin naturally occurring tricyclic aromatic compound, efficiently inhibited lytic replication of MHV-68 in a dose-dependent manner following the virus entry. The IC50 of angelicin antiviral activity was estimated to be 28.95 μM, while the CC50 of angelicin was higher than 2600 μM. Furthermore, incubation with angelicin efficiently inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced lytic replication of human gammaherpresviruses in both EBV- and KSHV-infected cells. Taken together, these results suggest that MHV-68 can be a useful tool to screen novel antiviral agents against human gammaherepsviruses and that angelicin may provide a lead structure for the development of antiviral drug against gammaherpesviruses.",

keywords = "Angelicin, Antiviral activity, EBV, Furocoumarins, KSHV, MHV-68",

author = "Cho, {Hye Jeong} and Jeong, {Seon Gyeong} and Park, {Ji Eun} and Han, {Jin Ah} and Kang, {Hye Ri} and Dongho Lee and Song, {Moon Jung}",

note = "Funding Information: This study was supported by a Korea University Grant, a Grant of the Korea Healthcare technology R&D Project, Ministry of Health, Welfare & Family Affairs, Republic of Korea (A091021), the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MEST) (2008-2005321) and Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries (2011–2013), Republic of Korea to M.J.S.",

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AU - Cho, Hye Jeong

AU - Jeong, Seon Gyeong

AU - Park, Ji Eun

AU - Han, Jin Ah

AU - Kang, Hye Ri

AU - Lee, Dongho

AU - Song, Moon Jung

N1 - Funding Information: This study was supported by a Korea University Grant, a Grant of the Korea Healthcare technology R&D Project, Ministry of Health, Welfare & Family Affairs, Republic of Korea (A091021), the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MEST) (2008-2005321) and Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries (2011–2013), Republic of Korea to M.J.S.

PY - 2013

Y1 - 2013

N2 - Human gammaherpesviruses including Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are important pathogens as they persist in the host and cause various malignancies. However, few antiviral drugs are available to efficiently control gammaherpesvirus replication. Here we identified the antiviral activity of angelicin against murine gammaherpesvirus 68 (MHV-68), genetically and biologically related to human gammaherpesviruses. Angelicin, a furocoumarin naturally occurring tricyclic aromatic compound, efficiently inhibited lytic replication of MHV-68 in a dose-dependent manner following the virus entry. The IC50 of angelicin antiviral activity was estimated to be 28.95 μM, while the CC50 of angelicin was higher than 2600 μM. Furthermore, incubation with angelicin efficiently inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced lytic replication of human gammaherpresviruses in both EBV- and KSHV-infected cells. Taken together, these results suggest that MHV-68 can be a useful tool to screen novel antiviral agents against human gammaherepsviruses and that angelicin may provide a lead structure for the development of antiviral drug against gammaherpesviruses.

AB - Human gammaherpesviruses including Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are important pathogens as they persist in the host and cause various malignancies. However, few antiviral drugs are available to efficiently control gammaherpesvirus replication. Here we identified the antiviral activity of angelicin against murine gammaherpesvirus 68 (MHV-68), genetically and biologically related to human gammaherpesviruses. Angelicin, a furocoumarin naturally occurring tricyclic aromatic compound, efficiently inhibited lytic replication of MHV-68 in a dose-dependent manner following the virus entry. The IC50 of angelicin antiviral activity was estimated to be 28.95 μM, while the CC50 of angelicin was higher than 2600 μM. Furthermore, incubation with angelicin efficiently inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced lytic replication of human gammaherpresviruses in both EBV- and KSHV-infected cells. Taken together, these results suggest that MHV-68 can be a useful tool to screen novel antiviral agents against human gammaherepsviruses and that angelicin may provide a lead structure for the development of antiviral drug against gammaherpesviruses.

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Antiviral activity of angelicin against gammaherpesviruses

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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