Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle

Hye Rim Suh; Eui Ho Park; Sun Wook Moon; Ji Won Kim; Hwi Young Cho; Hee Chul Han

doi:10.1002/mus.26359

Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle

Hye Rim Suh, Eui Ho Park, Sun Wook Moon, Ji Won Kim, Hwi Young Cho, Hee Chul Han

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve 59:263–269, 2019.

Original language	English
Pages (from-to)	263-269
Number of pages	7
Journal	Muscle and Nerve
Volume	59
Issue number	2
DOIs	https://doi.org/10.1002/mus.26359
Publication status	Published - 2019 Feb

Bibliographical note

Funding Information:
Abbreviations: Acyp2, acylphosphatase-2; ANOVA, analysis of variance; BAX, pro-apoptotic Bcl-2 family member; CONT, naïve rats; DAPI, 4′,6-diamidine-2-phenylidole-dihydrochloride; HDAC6, histone deacety-lase 6; INrf2, Kelch-like ECH-associated protein 1; LENG, lengthened immobilization; MYH, myosin heavy chain; MyoD, myogenic differentiation factor D; NEUT, neutral angle (= 0°) immobilization; PARP, poly (ADP-ribose)polymerase; PBS, phosphate-buffered saline; RT, room temperature; SLC25A4, solute carrier family 25, member 4; TUNEL, terminal deoxynucleotidyl transferase (TdT) –mediated deoxyuridinetriphosphate (dUTP) nick end-labeling Key words: apoptosis, Bax, casting, lengthened immobilization, lengthening, soleus Funding: This research was supported by grants from the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund (Grant 20140101) and the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (Grant 2017R1D1A1B03030568 to H.-R.S.).

Publisher Copyright:
© 2018 Wiley Periodicals, Inc.

Keywords

Bax
apoptosis
casting
lengthened immobilization
lengthening
soleus

ASJC Scopus subject areas

Physiology
Clinical Neurology
Cellular and Molecular Neuroscience
Physiology (medical)

Access to Document

10.1002/mus.26359

Cite this

@article{fafbd853b34045f9a14b156fcf946a9a,

title = "Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle",

abstract = "Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve 59:263–269, 2019.",

keywords = "Bax, apoptosis, casting, lengthened immobilization, lengthening, soleus",

author = "Suh, {Hye Rim} and Park, {Eui Ho} and Moon, {Sun Wook} and Kim, {Ji Won} and Cho, {Hwi Young} and Han, {Hee Chul}",

note = "Funding Information: Abbreviations: Acyp2, acylphosphatase-2; ANOVA, analysis of variance; BAX, pro-apoptotic Bcl-2 family member; CONT, na{\"i}ve rats; DAPI, 4′,6-diamidine-2-phenylidole-dihydrochloride; HDAC6, histone deacety-lase 6; INrf2, Kelch-like ECH-associated protein 1; LENG, lengthened immobilization; MYH, myosin heavy chain; MyoD, myogenic differentiation factor D; NEUT, neutral angle (= 0°) immobilization; PARP, poly (ADP-ribose)polymerase; PBS, phosphate-buffered saline; RT, room temperature; SLC25A4, solute carrier family 25, member 4; TUNEL, terminal deoxynucleotidyl transferase (TdT) –mediated deoxyuridinetriphosphate (dUTP) nick end-labeling Key words: apoptosis, Bax, casting, lengthened immobilization, lengthening, soleus Funding: This research was supported by grants from the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund (Grant 20140101) and the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (Grant 2017R1D1A1B03030568 to H.-R.S.). Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",

year = "2019",

month = feb,

doi = "10.1002/mus.26359",

language = "English",

volume = "59",

pages = "263--269",

journal = "Muscle and Nerve",

issn = "0148-639X",

publisher = "John Wiley & Sons Inc.",

number = "2",

}

TY - JOUR

T1 - Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle

AU - Suh, Hye Rim

AU - Park, Eui Ho

AU - Moon, Sun Wook

AU - Kim, Ji Won

AU - Cho, Hwi Young

AU - Han, Hee Chul

N1 - Funding Information: Abbreviations: Acyp2, acylphosphatase-2; ANOVA, analysis of variance; BAX, pro-apoptotic Bcl-2 family member; CONT, naïve rats; DAPI, 4′,6-diamidine-2-phenylidole-dihydrochloride; HDAC6, histone deacety-lase 6; INrf2, Kelch-like ECH-associated protein 1; LENG, lengthened immobilization; MYH, myosin heavy chain; MyoD, myogenic differentiation factor D; NEUT, neutral angle (= 0°) immobilization; PARP, poly (ADP-ribose)polymerase; PBS, phosphate-buffered saline; RT, room temperature; SLC25A4, solute carrier family 25, member 4; TUNEL, terminal deoxynucleotidyl transferase (TdT) –mediated deoxyuridinetriphosphate (dUTP) nick end-labeling Key words: apoptosis, Bax, casting, lengthened immobilization, lengthening, soleus Funding: This research was supported by grants from the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund (Grant 20140101) and the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (Grant 2017R1D1A1B03030568 to H.-R.S.). Publisher Copyright: © 2018 Wiley Periodicals, Inc.

PY - 2019/2

Y1 - 2019/2

N2 - Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve 59:263–269, 2019.

AB - Introduction: Lengthened immobilization may prevent muscle shortening, and help maintain normal muscle length. However, its apoptotic effects remain unclear. We evaluated the effects of long-term immobilization on apoptotic proteins. Methods: Rat soleus muscles were immobilized by casting in a neutral (NEUT) or lengthened (LENG) position for 21 days. We evaluated dynamic weight load and muscle atrophy following the 21-day period using hematoxylin and eosin staining. We measured Bax (pro-apoptotic Bcl-2 family member), MyoD (myogenic differentiation factor D), MYH (myosin heavy chain), and cleaved poly(ADP-ribose)polymerase levels and examined apoptotic nucleus expression. Results: Decreased dynamic weight load and muscle atrophy changes were observed in LENG. Both NEUT and LENG showed significantly reduced levels of MYH. LENG showed a significant increase in Bax and MyoD expression as well as in the number of apoptotic nuclei. Conclusions: Long-term lengthened immobilization may increase apoptotic changes and decrease muscle formation proteins in muscle. Muscle Nerve 59:263–269, 2019.

KW - Bax

KW - apoptosis

KW - casting

KW - lengthened immobilization

KW - lengthening

KW - soleus

UR - http://www.scopus.com/inward/record.url?scp=85058777077&partnerID=8YFLogxK

U2 - 10.1002/mus.26359

DO - 10.1002/mus.26359

M3 - Article

C2 - 30338859

AN - SCOPUS:85058777077

SN - 0148-639X

VL - 59

SP - 263

EP - 269

JO - Muscle and Nerve

JF - Muscle and Nerve

IS - 2

ER -

Apoptotic changes in a full-lengthened immobilization model of rat soleus muscle

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this