Application of electrical treatment on Euglena gracilis for increasing paramylon production

Jee Young Kim, Jeong Joo Oh, Da Hee Kim, Hyun Soo Kim, Changsu Lee, Jaewon Park, Yoon E. Choi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Abstract: Paramylon also called β-1,3-glucan is a value-added product produced from Euglena gracilis. Recently, researchers have developed various strategies for the enhanced paramylon production, among which electrical treatment for microbial stimulation can be an alternative owing to the applicability to large-scale cultivation. In this study, we applied the electrical treatment for enhanced paramylon production and found the proper treatment conditions. Under the treatment with platinum electrodes at 10 mA, the paramylon production of treated cells was significantly increased about 2.5-fold, compared to those of the untreated cells, although the density of cells was maintained due to considerable stress. The size of treated cells became larger, possibly due to the increased level of paramylon production within the cells. Accordingly, the contents of glucose uptake, glucose-6-phosphate (G6P), glucose-1-phosphate (G1P), and uridine diphosphoglucose (UDPG) were shifted to appropriate states for the process of paramylon synthesis under the treatment. The increased level of transcripts encoding glucan synthase-like 2 (EgGSL2) was also confirmed via droplet digital PCR (ddPCR) under the treatment. Overall, this study makes a major contribution to research on electrical stimulation and provides new insights into E. gracilis metabolism like paramylon synthesis. Key points: • Electrical treatment induced the paramylon production and morphological change of Euglena gracilis. • The glucose uptake of E. gracilis was increased during the electrical treatment, fueling the paramylon synthesis.

Original languageEnglish
Pages (from-to)1031-1039
Number of pages9
JournalApplied Microbiology and Biotechnology
Issue number3
Publication statusPublished - 2021 Feb


  • Electrical stimulation
  • Euglena gracilis
  • Paramylon
  • ddPCR and beta glucan synthases

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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