Application of fluorescent turn-on aptamers in RNA studies

Mohammad Faysal Al Mazid, Olha Shkel, Yevheniia Kharkivska, Jun Seok Lee

Research output: Contribution to journalReview articlepeer-review

2 Citations (Scopus)

Abstract

RNA is an intermediate player between DNA transcription and protein translation. RNAs also interact with other macromolecules and metabolites and regulate their fate. The emerging number of RNA identifications expanded new areas of study to determine their applicability and functional analysis. Recently, extensive research has been focused on visualizing RNA in living biological samples and a method has been developed by the evolution of specific fluorophore-binding aptamers through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method. Several promising fluorescent turn-on aptamers are currently available, and they can detect RNA-RNA, RNA-protein, ligand binding, small molecule, and metabolite interactionsin vitroand under live-cell conditions. Here we review the currently available fluorescent turn-on aptamers and discuss their applicability for analyzing the fate of targeted RNAs inin vitroandin vivosystems.

Original languageEnglish
Pages (from-to)483-491
Number of pages9
JournalMolecular Omics
Volume17
Issue number4
DOIs
Publication statusPublished - 2021 Aug

Bibliographical note

Funding Information:
This work is supported by the National Research Foundation funded by the Ministry of Science, ICT & Future Planning (NRF-2018M3A9H4079286 and NRF-2020R1A2C2004422).

Publisher Copyright:
© The Royal Society of Chemistry 2021.

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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