Application of high throughput cell array technology to FISH: Investigation of the role of deletion of p16 gene in leukemias

Dong Soon Lee, Jee Hyung Lee, Hyun Chung Min, Tae Young Kim, Bo Ra Oh, Ho Young Kim, Jong Yun Lee, Chung Kee Lee, Hong Gu Chun, Hee Chan Kim

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Bio-cell chip is a chip that has hundreds of types of cells arrayed and immobilized on a small slide. To elucidate the role of deletion of the p16 gene in hematologic malignancies, the bio-cell chip technique was applied to fluorescent in situ hybridization (FISH) study. We made a bio-cell chip with bone marrow specimen from 109 patients with acute lymphoblastic leukemia (ALL), 102 patients with acute myelogenous leukemia (AML), 47 patients with chronic myelogenous leukemia (CML), and 25 patients with multiple myeloma (MM). A glass slide with 96 separated areas was fabricated, onto which was added methanol/acetic acid fixed cell suspensions for high-throughput FISH for p16. With the successful application of bio-cell chip technique, we found that the deletion of p16 contributed to the oncogenesis in acute leukemia, but not in chronic leukemia. In conclusion, the bio-cell chip, a cell version of ultrahigh-throughput technology, was successfully applied to the FISH study, which can be utilized efficiently in the molecular cytogenetic investigation of hematologic malignancies.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalJournal of Biotechnology
Issue number3
Publication statusPublished - 2007 Jan 10
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by Ministry of Health and Welfare, Republic of Korea (A06-0423) and National Research Laboratory Program of The Ministry of Science and Technology (M1-0302-00-0112).


  • Bio-cell chip
  • Deletion
  • FISH
  • p16

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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