Application of M1 macrophage as a live vector in delivering nanoparticles for in vivo photothermal treatment

Nu Ri Im; Taeseok Daniel Yang; Kwanjun Park; Jang Hoon Lee; Jonghwan Lee; Yoon Hyuck Kim; Jae-Seung Lee; Byoungjae Kim; Kwang-Yoon Jung; Youngwoon Choi; Seung Kuk Baek

doi:10.1016/j.jare.2021.01.010

Application of M1 macrophage as a live vector in delivering nanoparticles for in vivo photothermal treatment

Nu Ri Im, Taeseok Daniel Yang, Kwanjun Park, Jang Hoon Lee, Jonghwan Lee, Yoon Hyuck Kim, Jae-Seung Lee, Byoungjae Kim, Kwang-Yoon Jung, Youngwoon Choi, Seung Kuk Baek

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Introduction: To enhance photothermal treatment (PTT) efficiency, a delivery method that uses cell vector for nanoparticles (NPs) delivery has drawn attention and studied widely in recent years. Objectives: In this study, we demonstrated the feasibility of M1 activated macrophage as a live vector for delivering NPs and investigated the effect of NPs loaded M1 stimulated by Lipopolysaccharide on PTT efficiency in vivo. Methods: M1 was used as a live vector for delivering NPs and further to investigate the effect of NPs loaded M1 on PTT efficiency. Non-activated macrophage (MФ) was stimulated by lipopolysaccharide (LPS) into M1 and assessed for tumor cell phagocytic capacity towards NPs Results: We found M1 exhibited a 20-fold higher uptake capacity of NPs per cell volume and 2.9-fold more active infiltration into the tumor site, compared with non-activated macrophage MФ. We injected M1 cells peritumorally and observed that these cells penetrated into the tumor mass within 12 h. Then, we conducted PTT using irradiation of a near-infrared laser for 1 min at 1 W/cm². As a result, we confirmed that using M1 as an active live vector led to a more rapid reduction in tumor size within 1 day indicating that the efficacy of PTT with NPs-loaded M1 is higher than that with NPs-loaded MФ. Conclusion: Our study demonstrated the potential role of M1 as a live vector for enhancing the feasibility of PTT in cancer treatment.

Original language	English
Pages (from-to)	155-163
Number of pages	9
Journal	Journal of Advanced Research
Volume	31
DOIs	https://doi.org/10.1016/j.jare.2021.01.010
Publication status	Published - 2021 Jul

Bibliographical note

Funding Information:
We thank Dr. Chang-Min Lee for helpful comments and discussions. This research was supported by the Clinical Trial Center of Korea University Anam Hospital (I1500931), the Korea Health Technology R&D Project (HI14C0748) through the Korea Health Industry Development Institute (KHIDI) by the Ministry of Health & Welfare, and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2016R1D1A1A02937362, NRF-2018R1D1A1A09083263, NRF-2019R1A2C4004804, and NRF-2019H1A2A1076334), and National Eye Institute (R01EY030569). Institute of Information and Communications Technology Planning and Evaluation (IITP; MSIT) (2020-0-00997). This research was also supported by a grant from Korea University.

Funding Information:
We thank Dr. Chang-Min Lee for helpful comments and discussions. This research was supported by the Clinical Trial Center of Korea University Anam Hospital (I1500931), the Korea Health Technology R&D Project (HI14C0748) through the Korea Health Industry Development Institute (KHIDI) by the Ministry of Health & Welfare, and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2016R1D1A1A02937362, NRF-2018R1D1A1A09083263, NRF-2019R1A2C4004804, and NRF-2019H1A2A1076334), and National Eye Institute (R01EY030569). Institute of Information and Communications Technology Planning and Evaluation (IITP; MSIT) (2020-0-00997). This research was also supported by a grant from Korea University. All institutional and national guidelines for the care and use of animals (The Korea University Institutional Animal Care and Use Committee) were followed.

Publisher Copyright:
© 2021

Keywords

Cancer therapy
Live cell vector
M1 macrophage
Medical optics and biotechnology
Photothermal effect
Photothermal treatment

ASJC Scopus subject areas

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.jare.2021.01.010

Cite this

@article{d5a11a8fbb24426eb40738077eecb522,

title = "Application of M1 macrophage as a live vector in delivering nanoparticles for in vivo photothermal treatment",

abstract = "Introduction: To enhance photothermal treatment (PTT) efficiency, a delivery method that uses cell vector for nanoparticles (NPs) delivery has drawn attention and studied widely in recent years. Objectives: In this study, we demonstrated the feasibility of M1 activated macrophage as a live vector for delivering NPs and investigated the effect of NPs loaded M1 stimulated by Lipopolysaccharide on PTT efficiency in vivo. Methods: M1 was used as a live vector for delivering NPs and further to investigate the effect of NPs loaded M1 on PTT efficiency. Non-activated macrophage (MФ) was stimulated by lipopolysaccharide (LPS) into M1 and assessed for tumor cell phagocytic capacity towards NPs Results: We found M1 exhibited a 20-fold higher uptake capacity of NPs per cell volume and 2.9-fold more active infiltration into the tumor site, compared with non-activated macrophage MФ. We injected M1 cells peritumorally and observed that these cells penetrated into the tumor mass within 12 h. Then, we conducted PTT using irradiation of a near-infrared laser for 1 min at 1 W/cm2. As a result, we confirmed that using M1 as an active live vector led to a more rapid reduction in tumor size within 1 day indicating that the efficacy of PTT with NPs-loaded M1 is higher than that with NPs-loaded MФ. Conclusion: Our study demonstrated the potential role of M1 as a live vector for enhancing the feasibility of PTT in cancer treatment.",

keywords = "Cancer therapy, Live cell vector, M1 macrophage, Medical optics and biotechnology, Photothermal effect, Photothermal treatment",

author = "Im, {Nu Ri} and Yang, {Taeseok Daniel} and Kwanjun Park and Lee, {Jang Hoon} and Jonghwan Lee and {Hyuck Kim}, Yoon and Jae-Seung Lee and Byoungjae Kim and Kwang-Yoon Jung and Youngwoon Choi and Baek, {Seung Kuk}",

note = "Funding Information: We thank Dr. Chang-Min Lee for helpful comments and discussions. This research was supported by the Clinical Trial Center of Korea University Anam Hospital (I1500931), the Korea Health Technology R&D Project (HI14C0748) through the Korea Health Industry Development Institute (KHIDI) by the Ministry of Health & Welfare, and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2016R1D1A1A02937362, NRF-2018R1D1A1A09083263, NRF-2019R1A2C4004804, and NRF-2019H1A2A1076334), and National Eye Institute (R01EY030569). Institute of Information and Communications Technology Planning and Evaluation (IITP; MSIT) (2020-0-00997). This research was also supported by a grant from Korea University. Funding Information: We thank Dr. Chang-Min Lee for helpful comments and discussions. This research was supported by the Clinical Trial Center of Korea University Anam Hospital (I1500931), the Korea Health Technology R&D Project (HI14C0748) through the Korea Health Industry Development Institute (KHIDI) by the Ministry of Health & Welfare, and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2016R1D1A1A02937362, NRF-2018R1D1A1A09083263, NRF-2019R1A2C4004804, and NRF-2019H1A2A1076334), and National Eye Institute (R01EY030569). Institute of Information and Communications Technology Planning and Evaluation (IITP; MSIT) (2020-0-00997). This research was also supported by a grant from Korea University. All institutional and national guidelines for the care and use of animals (The Korea University Institutional Animal Care and Use Committee) were followed. Publisher Copyright: {\textcopyright} 2021",

year = "2021",

month = jul,

doi = "10.1016/j.jare.2021.01.010",

language = "English",

volume = "31",

pages = "155--163",

journal = "Journal of Advanced Research",

issn = "2090-1232",

publisher = "Cairo University",

}

TY - JOUR

T1 - Application of M1 macrophage as a live vector in delivering nanoparticles for in vivo photothermal treatment

AU - Im, Nu Ri

AU - Yang, Taeseok Daniel

AU - Park, Kwanjun

AU - Lee, Jang Hoon

AU - Lee, Jonghwan

AU - Hyuck Kim, Yoon

AU - Lee, Jae-Seung

AU - Kim, Byoungjae

AU - Jung, Kwang-Yoon

AU - Choi, Youngwoon

AU - Baek, Seung Kuk

N1 - Funding Information: We thank Dr. Chang-Min Lee for helpful comments and discussions. This research was supported by the Clinical Trial Center of Korea University Anam Hospital (I1500931), the Korea Health Technology R&D Project (HI14C0748) through the Korea Health Industry Development Institute (KHIDI) by the Ministry of Health & Welfare, and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2016R1D1A1A02937362, NRF-2018R1D1A1A09083263, NRF-2019R1A2C4004804, and NRF-2019H1A2A1076334), and National Eye Institute (R01EY030569). Institute of Information and Communications Technology Planning and Evaluation (IITP; MSIT) (2020-0-00997). This research was also supported by a grant from Korea University. Funding Information: We thank Dr. Chang-Min Lee for helpful comments and discussions. This research was supported by the Clinical Trial Center of Korea University Anam Hospital (I1500931), the Korea Health Technology R&D Project (HI14C0748) through the Korea Health Industry Development Institute (KHIDI) by the Ministry of Health & Welfare, and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2016R1D1A1A02937362, NRF-2018R1D1A1A09083263, NRF-2019R1A2C4004804, and NRF-2019H1A2A1076334), and National Eye Institute (R01EY030569). Institute of Information and Communications Technology Planning and Evaluation (IITP; MSIT) (2020-0-00997). This research was also supported by a grant from Korea University. All institutional and national guidelines for the care and use of animals (The Korea University Institutional Animal Care and Use Committee) were followed. Publisher Copyright: © 2021

PY - 2021/7

Y1 - 2021/7

N2 - Introduction: To enhance photothermal treatment (PTT) efficiency, a delivery method that uses cell vector for nanoparticles (NPs) delivery has drawn attention and studied widely in recent years. Objectives: In this study, we demonstrated the feasibility of M1 activated macrophage as a live vector for delivering NPs and investigated the effect of NPs loaded M1 stimulated by Lipopolysaccharide on PTT efficiency in vivo. Methods: M1 was used as a live vector for delivering NPs and further to investigate the effect of NPs loaded M1 on PTT efficiency. Non-activated macrophage (MФ) was stimulated by lipopolysaccharide (LPS) into M1 and assessed for tumor cell phagocytic capacity towards NPs Results: We found M1 exhibited a 20-fold higher uptake capacity of NPs per cell volume and 2.9-fold more active infiltration into the tumor site, compared with non-activated macrophage MФ. We injected M1 cells peritumorally and observed that these cells penetrated into the tumor mass within 12 h. Then, we conducted PTT using irradiation of a near-infrared laser for 1 min at 1 W/cm2. As a result, we confirmed that using M1 as an active live vector led to a more rapid reduction in tumor size within 1 day indicating that the efficacy of PTT with NPs-loaded M1 is higher than that with NPs-loaded MФ. Conclusion: Our study demonstrated the potential role of M1 as a live vector for enhancing the feasibility of PTT in cancer treatment.

AB - Introduction: To enhance photothermal treatment (PTT) efficiency, a delivery method that uses cell vector for nanoparticles (NPs) delivery has drawn attention and studied widely in recent years. Objectives: In this study, we demonstrated the feasibility of M1 activated macrophage as a live vector for delivering NPs and investigated the effect of NPs loaded M1 stimulated by Lipopolysaccharide on PTT efficiency in vivo. Methods: M1 was used as a live vector for delivering NPs and further to investigate the effect of NPs loaded M1 on PTT efficiency. Non-activated macrophage (MФ) was stimulated by lipopolysaccharide (LPS) into M1 and assessed for tumor cell phagocytic capacity towards NPs Results: We found M1 exhibited a 20-fold higher uptake capacity of NPs per cell volume and 2.9-fold more active infiltration into the tumor site, compared with non-activated macrophage MФ. We injected M1 cells peritumorally and observed that these cells penetrated into the tumor mass within 12 h. Then, we conducted PTT using irradiation of a near-infrared laser for 1 min at 1 W/cm2. As a result, we confirmed that using M1 as an active live vector led to a more rapid reduction in tumor size within 1 day indicating that the efficacy of PTT with NPs-loaded M1 is higher than that with NPs-loaded MФ. Conclusion: Our study demonstrated the potential role of M1 as a live vector for enhancing the feasibility of PTT in cancer treatment.

KW - Cancer therapy

KW - Live cell vector

KW - M1 macrophage

KW - Medical optics and biotechnology

KW - Photothermal effect

KW - Photothermal treatment

UR - http://www.scopus.com/inward/record.url?scp=85101341124&partnerID=8YFLogxK

U2 - 10.1016/j.jare.2021.01.010

DO - 10.1016/j.jare.2021.01.010

M3 - Article

AN - SCOPUS:85101341124

SN - 2090-1232

VL - 31

SP - 155

EP - 163

JO - Journal of Advanced Research

JF - Journal of Advanced Research

ER -

Application of M1 macrophage as a live vector in delivering nanoparticles for in vivo photothermal treatment

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this