Application of tissue microarray for atherectomized tissues from peripheral arterial disease

Se Hoon Kim; Dong Min Kim; Hyosup Shim; Junjeong Choi; Seong Hwan Park; Seung Min Song; Young Ho Shin; Donghoon Choi

doi:10.1016/j.prp.2011.06.007

Application of tissue microarray for atherectomized tissues from peripheral arterial disease

Se Hoon Kim, Dong Min Kim, Hyosup Shim, Junjeong Choi, Seong Hwan Park, Seung Min Song, Young Ho Shin, Donghoon Choi

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

It is not easy to apply tissue microarray (TMA) to atherectomized tissues from peripheral arterial disease because of their physical properties. We introduce a new TMA application technique for atherectomized tissues. Using a pre-made plastic TMA cassette and TMA punch device, we successfully made the TMA block containing 40 vertically oriented atherectomized tissue samples from 10 patients. The histogram of surface areas of tissue cores in the TMA showed a bell-shaped distribution, whereas that of conventionally embedded tissues showed wide distribution. This finding suggests that the TMA method might be a better way of vertical embedding than the conventional method. A TMA block prepared by our method enabled a simultaneous evaluation of the histopathology of vertically oriented atherectomized tissues and the correlation between them with intravascular ultrasound image. In addition, this new method might be applied to various tissues in different ways.

Original language	English
Pages (from-to)	568-572
Number of pages	5
Journal	Pathology Research and Practice
Volume	207
Issue number	9
DOIs	https://doi.org/10.1016/j.prp.2011.06.007
Publication status	Published - 2011 Sept 15
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea Health 21 R&D Project under the Ministry of Health & Welfare of the Republic of Korea (No. A085136 ).

Keywords

Atherectomy
Intravascular ultrasound
Peripheral arterial disease
Tissue microarray

ASJC Scopus subject areas

Pathology and Forensic Medicine
Cell Biology

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10.1016/j.prp.2011.06.007

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title = "Application of tissue microarray for atherectomized tissues from peripheral arterial disease",

abstract = "It is not easy to apply tissue microarray (TMA) to atherectomized tissues from peripheral arterial disease because of their physical properties. We introduce a new TMA application technique for atherectomized tissues. Using a pre-made plastic TMA cassette and TMA punch device, we successfully made the TMA block containing 40 vertically oriented atherectomized tissue samples from 10 patients. The histogram of surface areas of tissue cores in the TMA showed a bell-shaped distribution, whereas that of conventionally embedded tissues showed wide distribution. This finding suggests that the TMA method might be a better way of vertical embedding than the conventional method. A TMA block prepared by our method enabled a simultaneous evaluation of the histopathology of vertically oriented atherectomized tissues and the correlation between them with intravascular ultrasound image. In addition, this new method might be applied to various tissues in different ways.",

keywords = "Atherectomy, Intravascular ultrasound, Peripheral arterial disease, Tissue microarray",

author = "Kim, {Se Hoon} and Kim, {Dong Min} and Hyosup Shim and Junjeong Choi and Park, {Seong Hwan} and Song, {Seung Min} and Shin, {Young Ho} and Donghoon Choi",

note = "Funding Information: This study was supported by a grant from the Korea Health 21 R&D Project under the Ministry of Health & Welfare of the Republic of Korea (No. A085136 ).",

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doi = "10.1016/j.prp.2011.06.007",

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AU - Kim, Se Hoon

AU - Kim, Dong Min

AU - Shim, Hyosup

AU - Choi, Junjeong

AU - Park, Seong Hwan

AU - Song, Seung Min

AU - Shin, Young Ho

AU - Choi, Donghoon

N1 - Funding Information: This study was supported by a grant from the Korea Health 21 R&D Project under the Ministry of Health & Welfare of the Republic of Korea (No. A085136 ).

PY - 2011/9/15

Y1 - 2011/9/15

N2 - It is not easy to apply tissue microarray (TMA) to atherectomized tissues from peripheral arterial disease because of their physical properties. We introduce a new TMA application technique for atherectomized tissues. Using a pre-made plastic TMA cassette and TMA punch device, we successfully made the TMA block containing 40 vertically oriented atherectomized tissue samples from 10 patients. The histogram of surface areas of tissue cores in the TMA showed a bell-shaped distribution, whereas that of conventionally embedded tissues showed wide distribution. This finding suggests that the TMA method might be a better way of vertical embedding than the conventional method. A TMA block prepared by our method enabled a simultaneous evaluation of the histopathology of vertically oriented atherectomized tissues and the correlation between them with intravascular ultrasound image. In addition, this new method might be applied to various tissues in different ways.

AB - It is not easy to apply tissue microarray (TMA) to atherectomized tissues from peripheral arterial disease because of their physical properties. We introduce a new TMA application technique for atherectomized tissues. Using a pre-made plastic TMA cassette and TMA punch device, we successfully made the TMA block containing 40 vertically oriented atherectomized tissue samples from 10 patients. The histogram of surface areas of tissue cores in the TMA showed a bell-shaped distribution, whereas that of conventionally embedded tissues showed wide distribution. This finding suggests that the TMA method might be a better way of vertical embedding than the conventional method. A TMA block prepared by our method enabled a simultaneous evaluation of the histopathology of vertically oriented atherectomized tissues and the correlation between them with intravascular ultrasound image. In addition, this new method might be applied to various tissues in different ways.

KW - Atherectomy

KW - Intravascular ultrasound

KW - Peripheral arterial disease

KW - Tissue microarray

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Application of tissue microarray for atherectomized tissues from peripheral arterial disease

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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