Aptamer biosensor for lable-free detection of human immunoglobulin E based on surface plasmon resonance

Yun Hwa Kim; Jun Pyo Kim; Se Jong Han; Sang Jun Sim

doi:10.1016/j.snb.2009.03.013

Aptamer biosensor for lable-free detection of human immunoglobulin E based on surface plasmon resonance

Yun Hwa Kim, Jun Pyo Kim, Se Jong Han, Sang Jun Sim

Research output: Contribution to journal › Article › peer-review

64 Citations (Scopus)

Abstract

Aptasensor, an aptamer-based sensor for non-labeled detection of human immunoglobulin E (IgE), was developed using surface plasmon resonance (SPR). Various oligo(ethylene glycol) mixtures of different molar ratios of EG₆-COOH and EG₃-OH were used for the construction of self-assembled monolayers (SAM). Direct assays and modified sandwich assays over a 1.0 nM anti-IgE, aptamer-immobilized, gold chip, consisting of a 1:3 molar ratio of EG₆-COOH:EG₃-OH SAM, were investigated for real-time IgE detection. The linear ranges for both were 1.0-1000 ng/mL and the lowest amounts of detectable analytes in the samples were 3.44 and 2.07 ng/mL for direct and modified sandwich assays, respectively. These results for IgE detection suggest that the aptamer offers the possibility of a simple and realistic biosensor platform based on a SAM-fabricated SPR system. Furthermore, as an optic-biosensor, an SPR aptasensor could be used for the diagnosis of allergies.

Original language	English
Pages (from-to)	471-475
Number of pages	5
Journal	Sensors and Actuators, B: Chemical
Volume	139
Issue number	2
DOIs	https://doi.org/10.1016/j.snb.2009.03.013
Publication status	Published - 2009 Jun 4
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program grant funded by the Korea government (MEST) (grant no. R0A-2008-000-20078-0) of the Republic of Korea.

Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.

Keywords

Aptamer
Immunoglobulin E (IgE)
Self-assembled monolayer (SAM)
Surface plasmon resonance (SPR)

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2009.03.013

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title = "Aptamer biosensor for lable-free detection of human immunoglobulin E based on surface plasmon resonance",

abstract = "Aptasensor, an aptamer-based sensor for non-labeled detection of human immunoglobulin E (IgE), was developed using surface plasmon resonance (SPR). Various oligo(ethylene glycol) mixtures of different molar ratios of EG6-COOH and EG3-OH were used for the construction of self-assembled monolayers (SAM). Direct assays and modified sandwich assays over a 1.0 nM anti-IgE, aptamer-immobilized, gold chip, consisting of a 1:3 molar ratio of EG6-COOH:EG3-OH SAM, were investigated for real-time IgE detection. The linear ranges for both were 1.0-1000 ng/mL and the lowest amounts of detectable analytes in the samples were 3.44 and 2.07 ng/mL for direct and modified sandwich assays, respectively. These results for IgE detection suggest that the aptamer offers the possibility of a simple and realistic biosensor platform based on a SAM-fabricated SPR system. Furthermore, as an optic-biosensor, an SPR aptasensor could be used for the diagnosis of allergies.",

keywords = "Aptamer, Immunoglobulin E (IgE), Self-assembled monolayer (SAM), Surface plasmon resonance (SPR)",

author = "Kim, {Yun Hwa} and Kim, {Jun Pyo} and Han, {Se Jong} and Sim, {Sang Jun}",

note = "Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program grant funded by the Korea government (MEST) (grant no. R0A-2008-000-20078-0) of the Republic of Korea. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

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AU - Han, Se Jong

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N1 - Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program grant funded by the Korea government (MEST) (grant no. R0A-2008-000-20078-0) of the Republic of Korea. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

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N2 - Aptasensor, an aptamer-based sensor for non-labeled detection of human immunoglobulin E (IgE), was developed using surface plasmon resonance (SPR). Various oligo(ethylene glycol) mixtures of different molar ratios of EG6-COOH and EG3-OH were used for the construction of self-assembled monolayers (SAM). Direct assays and modified sandwich assays over a 1.0 nM anti-IgE, aptamer-immobilized, gold chip, consisting of a 1:3 molar ratio of EG6-COOH:EG3-OH SAM, were investigated for real-time IgE detection. The linear ranges for both were 1.0-1000 ng/mL and the lowest amounts of detectable analytes in the samples were 3.44 and 2.07 ng/mL for direct and modified sandwich assays, respectively. These results for IgE detection suggest that the aptamer offers the possibility of a simple and realistic biosensor platform based on a SAM-fabricated SPR system. Furthermore, as an optic-biosensor, an SPR aptasensor could be used for the diagnosis of allergies.

AB - Aptasensor, an aptamer-based sensor for non-labeled detection of human immunoglobulin E (IgE), was developed using surface plasmon resonance (SPR). Various oligo(ethylene glycol) mixtures of different molar ratios of EG6-COOH and EG3-OH were used for the construction of self-assembled monolayers (SAM). Direct assays and modified sandwich assays over a 1.0 nM anti-IgE, aptamer-immobilized, gold chip, consisting of a 1:3 molar ratio of EG6-COOH:EG3-OH SAM, were investigated for real-time IgE detection. The linear ranges for both were 1.0-1000 ng/mL and the lowest amounts of detectable analytes in the samples were 3.44 and 2.07 ng/mL for direct and modified sandwich assays, respectively. These results for IgE detection suggest that the aptamer offers the possibility of a simple and realistic biosensor platform based on a SAM-fabricated SPR system. Furthermore, as an optic-biosensor, an SPR aptasensor could be used for the diagnosis of allergies.

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Aptamer biosensor for lable-free detection of human immunoglobulin E based on surface plasmon resonance

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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