Arabidopsis GDSL lipase 2 plays a role in pathogen defense via negative regulation of auxin signaling

Dong Sook Lee, Bo Kyung Kim, Sun Jae Kwon, Hak Chul Jin, Ohkmae K. Park

    Research output: Contribution to journalArticlepeer-review

    133 Citations (Scopus)

    Abstract

    GLIP1 was isolated previously from Arabidopsis, as a salicylic acid-responsive secreted GDSL lipase that functions in resistance to Alternaria brassicicola [I.S. Oh, A.R. Park, M.S. Bae, S.J. Kwon, Y.S. Kim, J.E. Lee, N.Y. Kang, S. Lee, H. Cheong, O.K. Park, Secretome analysis reveals an Arabidopsis lipase involved in defense against Alternaria brassicicola. Plant Cell 17 (2005) 2832-2847.]. To extend our knowledge of the roles played by GLIPs in Arabidopsis, we conducted functional studies of another family member, GLIP2. GLIP2 transcripts were expressed in young seedlings, as well as in the root and stem tissues of mature plants. GLIP2 transcript levels were elevated by treatment with salicylic acid, jasmonic acid and ethylene. Recombinant GLIP2 proteins possessed lipase and anti-microbial activities, inhibiting germination of fungal spores. In comparison to wild type plants, T-DNA insertion glip2 mutants exhibited enhanced auxin responses, including increased lateral root formation and elevated AUX/IAA gene expression. When challenged with the necrotropic bacteria Erwinia carotovora, glip2 mutants exhibited more susceptible phenotypes than wild type plants. These results suggest that GLIP2 plays a role in resistance to Erwinia carotovora via negative regulation of auxin signaling.

    Original languageEnglish
    Pages (from-to)1038-1042
    Number of pages5
    JournalBiochemical and biophysical research communications
    Volume379
    Issue number4
    DOIs
    Publication statusPublished - 2009 Feb 20

    Bibliographical note

    Funding Information:
    We thank Soon Il Kwon for technical comments. This work was supported by grants from the Plant Signaling Network Research Center (R11-2003-008-04004-0), the Biotechnology Development Program (2006-02762) funded by the Korea Science and Engineering Foundation and from the Basic Research Program (C00441) funded by the Korea Research Foundation.

    Keywords

    • Arabidopsis
    • Auxin signaling
    • Erwinia carotovora
    • GDSL lipase
    • GLIP2
    • Pathogen resistance

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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