Abstract
Flavonols are a class of flavonoids that are found in most plants. Certain flavonols exhibit anticancer, antioxidant, and antimicrobial functions. An array of genes plays a role in the regulation of flavonoid biosynthetic pathways, including the MYB–bHLH (basic helix-loop-helix-WD40 transcription factor complex. Flavonoids often display altered bioactivities after being glycosylated by the action of glycosyltransferases. These enzymes catalyze the transfer of sugars from a donor to various acceptors. In this study, we generated several transgenic lines of Arabidopsis that overexpress UDP-glycosyltransferase 78D1 (UGT78D1), which are hereafter referred to as UGT78D1-OX, to address three questions: (1) Can UGT78D1-OX seedlings accumulate more flavonols? (2) Can UGT78D1-OX seedlings accumulate more flavonols in the presence of sucrose? and (3) Will UGT78D1-OX be more sensitive to abiotic stresses? We observed that UGT78D1-OX seedlings accumulated specific types of kaempferol, while they had a decreased content of flavonols in the presence of sucrose. Contrary to our expectation, more anthocyanins accumulated in the UGT78D1-OX lines, although the expression of production of anthocyanin pigment 1 was slightly reduced in UGT78D1-OX seedlings compared with that in wild-type seedlings. It appeared that the overexpression of UGT78D1 did not interfere with abiotic stress tolerance in the mutant plants.
Original language | English |
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Pages (from-to) | 647-652 |
Number of pages | 6 |
Journal | Applied Biological Chemistry |
Volume | 60 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2017 Dec 1 |
Bibliographical note
Funding Information:Acknowledgments This work was supported by a grant from the National Research Foundation of Korea (Grant NRF-2017R1A2B4008706 to Hojoung Lee, 2017).
Publisher Copyright:
© 2017, The Korean Society for Applied Biological Chemistry.
Keywords
- Anthocyanin
- Arabidopsis
- Flavonoid
- Flavonol
- UDP-glycosyltransferase 78D1
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Organic Chemistry