Artificial Intelligence-Based Major Depressive Disorder (MDD) Diagnosis Using Raman Spectroscopic Features of Plasma Exosomes

Hyunku Shin, Youbin Kang, Kwan Woo Choi, Seungmin Kim, Byung Joo Ham, Yeonho Choi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


In vitro diagnosis using biomarkers for major depressive disorder (MDD) can offer considerable advantages in overcoming the lack of objective tests for depression and treating more patients. Plasma exosomes can be novel biomarkers for MDD based on their ability to pass through the blood-brain barrier and offer brain-related information. Here, we demonstrate a novel and precise MDD diagnosis using deep learning analysis and surface-enhanced Raman spectroscopy (SERS) of plasma exosomes. Our system is implemented based on 28,000 exosome SERS signals, providing sample-wise prediction results. Notably, this approach shows remarkable performance in predicting 70 test samples unused in the training step, with an area under the curve (AUC) of 0.939, a sensitivity of 91.4%, and a specificity of 88.6%. In addition, we confirm that the diagnostic scores were correlated with the degree of depression. These results show the utility of exosomes as novel biomarkers for MDD diagnosis and suggest a novel approach for prescreening techniques for psychiatric disorders.

Original languageEnglish
Pages (from-to)6410-6416
Number of pages7
JournalAnalytical chemistry
Issue number15
Publication statusPublished - 2023 Apr 18

Bibliographical note

Funding Information:
This research was supported by a grant from Seoul R&BD Program through the Seoul Business Agency (SBA) funded by the Seoul Metropolitan Government (BT210040, PI: H.S.) and the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety) (1711174279, RS-2020-KD000094, PI: Y.C.). This work was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2020M3E5D9080792; NRF-2022R1A2C2093009, PI: B.-J.H.). The authors used source images created by for and .

Publisher Copyright:
© 2023 American Chemical Society

ASJC Scopus subject areas

  • Analytical Chemistry


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