Abstract
The molecular players regulating the axon degeneration pathway have been identified using in vitro experimental models. Here, we describe an in vitro assay to assess the axonal fragmentation induced by mechanical injury to axons in cultured mouse embryonic dorsal root ganglion (DRG) neurons. DRG neurons are pseudounipolar and therefore suitable for an assay of axonal degeneration after injury. In addition, the time course of the axonal fragmentation is stereotyped, enabling the identification of reagents that either expedite or impede the degeneration process. With an image-based quantification method, the in vitro degeneration assay can be utilized as a platform supporting high-throughput screens for pharmacological or genetic reagents delaying axon degeneration.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 41-54 |
| Number of pages | 14 |
| DOIs | |
| Publication status | Published - 2020 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2143 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Bibliographical note
Funding Information:This work was supported by the National Research Foundation of Korea (NRF) grant to J.E.S. (NRF-2017R1C1B2008356), Health Technology R&D Project to Y.C. (HI17C1459), and BK21 Plus project of the NRF to J.E.S. and Y.C.
Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.
Keywords
- Axon degeneration
- Axotomy
- Dorsal root ganglion
- Image-based screening
- In vitro assay
- Neurodegeneration
ASJC Scopus subject areas
- Molecular Biology
- Genetics