Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy

Chung Chieh Yu; Condon Lau; James W. Tunnell; Martin Hunter; Maxim Kalashnikov; Christopher Fang-Yen; Stephen F. Fulghum; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld

doi:10.1364/OL.31.003119

Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy

Chung Chieh Yu, Condon Lau, James W. Tunnell, Martin Hunter, Maxim Kalashnikov, Christopher Fang-Yen, Stephen F. Fulghum, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

We describe azimuthal light scattering spectroscopy (φ/LSS), a novel technique for assessing epithelial-cell nuclear morphology. The difference between the spectra measured at azimuthal angles φ=0° and φ=90° preferentially isolates the single backscattering contribution due to large (∼10 μm) structures such as epithelial cell nuclei by discriminating against scattering from smaller organelles and diffusive background. We demonstrate the feasibility of using φ/LSS for cancer detection by showing that spectra from cancerous colon tissue exhibit significantly greater azimuthal asymmetry than spectra from normal colonic tissues.

Original language	English
Pages (from-to)	3119-3121
Number of pages	3
Journal	Optics Letters
Volume	31
Issue number	21
DOIs	https://doi.org/10.1364/OL.31.003119
Publication status	Published - 2006 Nov 1

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OL.31.003119

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title = "Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy",

abstract = "We describe azimuthal light scattering spectroscopy (φ/LSS), a novel technique for assessing epithelial-cell nuclear morphology. The difference between the spectra measured at azimuthal angles φ=0° and φ=90° preferentially isolates the single backscattering contribution due to large (∼10 μm) structures such as epithelial cell nuclei by discriminating against scattering from smaller organelles and diffusive background. We demonstrate the feasibility of using φ/LSS for cancer detection by showing that spectra from cancerous colon tissue exhibit significantly greater azimuthal asymmetry than spectra from normal colonic tissues.",

author = "Yu, {Chung Chieh} and Condon Lau and Tunnell, {James W.} and Martin Hunter and Maxim Kalashnikov and Christopher Fang-Yen and Fulghum, {Stephen F.} and Kamran Badizadegan and Dasari, {Ramachandra R.} and Feld, {Michael S.}",

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T1 - Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy

AU - Yu, Chung Chieh

AU - Lau, Condon

AU - Tunnell, James W.

AU - Hunter, Martin

AU - Kalashnikov, Maxim

AU - Fang-Yen, Christopher

AU - Fulghum, Stephen F.

AU - Badizadegan, Kamran

AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

PY - 2006/11/1

Y1 - 2006/11/1

N2 - We describe azimuthal light scattering spectroscopy (φ/LSS), a novel technique for assessing epithelial-cell nuclear morphology. The difference between the spectra measured at azimuthal angles φ=0° and φ=90° preferentially isolates the single backscattering contribution due to large (∼10 μm) structures such as epithelial cell nuclei by discriminating against scattering from smaller organelles and diffusive background. We demonstrate the feasibility of using φ/LSS for cancer detection by showing that spectra from cancerous colon tissue exhibit significantly greater azimuthal asymmetry than spectra from normal colonic tissues.

AB - We describe azimuthal light scattering spectroscopy (φ/LSS), a novel technique for assessing epithelial-cell nuclear morphology. The difference between the spectra measured at azimuthal angles φ=0° and φ=90° preferentially isolates the single backscattering contribution due to large (∼10 μm) structures such as epithelial cell nuclei by discriminating against scattering from smaller organelles and diffusive background. We demonstrate the feasibility of using φ/LSS for cancer detection by showing that spectra from cancerous colon tissue exhibit significantly greater azimuthal asymmetry than spectra from normal colonic tissues.

UR - http://www.scopus.com/inward/record.url?scp=33751327838&partnerID=8YFLogxK

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M3 - Article

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SN - 0146-9592

VL - 31

SP - 3119

EP - 3121

JO - Optics Letters

JF - Optics Letters

IS - 21

ER -

Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy

Abstract

ASJC Scopus subject areas

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