Assessment of adverse impacts of glyphosate-based herbicide, Excel Mera 71 by integrating multi-level biomarker responses in fishes

P. Samanta, S. Pal, A. K. Mukherjee, T. Senapati, J. Jung, A. R. Ghosh

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


This work was aimed to investigate the adverse impacts of Excel Mera 71 on Anabas testudineus, Heteropneustes fossilis and Oreochromis niloticus under laboratory (17.2 mg/L) and field (750 g/acre) conditions based on multiple-level integrated biomarker responses. Haematological parameters were influenced significantly and showed species specificity after Excel Mera 71 exposure under both the conditions. Physiological indices (hepato-somatic index and condition factor) showed higher values; however, hepato-somatic index induced significantly under both the conditions (p < 0.05). Total nuclear abnormalities at molecular level revealed enhanced level. Responses were more adverse in laboratory (O. niloticus > H. fossilis > A. testudineus), while total nuclear abnormalities under field showed the order as: H. fossilis (0.49 ± 0.08) > A. testudineus (0.46 ± 0.10) > O. niloticus (0.27 ± 0.05). Moreover, multi-level integrated biomarker responses displayed responses as: O. niloticus (6.97 fold) > H. fossilis (4.01 fold) > A. testudineus (2.33 fold) in laboratory; however, under field condition, responses were totally different [A. testudineus–H. fossilis (3.98 fold) > O. niloticus (3.01 fold)]. Additionally, glyphosate concentration was significantly correlated with several multi-level biomarker responses (p < 0.05). Moreover, multi-level integrated biomarker responses clearly indicated the higher impacts under laboratory experiment. Present investigation documented that multi-level integrative biomarker responses should be considered as reliable tool for assessment of herbicidal intoxication.

Original languageEnglish
Pages (from-to)6291-6300
Number of pages10
JournalInternational Journal of Environmental Science and Technology
Issue number10
Publication statusPublished - 2019 Oct 1

Bibliographical note

Funding Information:
Authors are thankful to Department of Science & Technology, India (DST/INSPIRE Fellowship/2011/164), for financial support. Presently, Dr Samanta joined Incheon National University as researcher. Authors are grateful to reviewers for improving the paper.

Publisher Copyright:
© 2018, Islamic Azad University (IAU).


  • Excel Mera 71
  • Genotoxicity
  • Haematology
  • Hepato-somatic index
  • Total nuclear abnormalities

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • General Agricultural and Biological Sciences


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