Assessment of the effect of cooking on speciation and bioaccessibility/cellular uptake of arsenic in rice, using in vitro digestion and Caco-2 and PSI cells as model

Seul Gi Lee; Jinhye Kim; Hyunjoon Park; Wilhelm Holzapfel; Kwang Won Lee

doi:10.1016/j.fct.2017.11.052

Assessment of the effect of cooking on speciation and bioaccessibility/cellular uptake of arsenic in rice, using in vitro digestion and Caco-2 and PSI cells as model

Seul Gi Lee, Jinhye Kim, Hyunjoon Park, Wilhelm Holzapfel, Kwang Won Lee

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

In vitro digestion/Caco-2 or pig small intestinal epithelium cell line (PSI) uptake models were used to study the bioaccessibility and cellular uptake of arsenic (As) in cooked white rice and brown rice. The arsenite(AsIII), was the predominant species in cooked rice and in its bioaccessible fractions. The percentage of total As bioaccessibility in white rice (75%) was slightly higher (p=0.061) than that in brown rice(66%). However, there was no difference in the inorganic As (iAs) bioaccessibility between white rice (95%) and brown rice (96%). In Caco-2 cell monolayer, total As retention was 7-31%, transport was 4-25%, and uptake (sum of retention and transport) was 16-38%. In PSI cell model, the retention, transport, and uptake of tAs were 10-28%, 14-31%, and 29-50%, respectively. In both cells, the cellular uptake of tAs in brown rice was 1.4-1.5 folds lower (p<0.05) than that of white rice. These results indicate that the cellular uptake of As can be affected by nutritional compositions. These in vitro screening methods can serve as preliminary screens to predict the relative impact in rice matrix having different As species and processing conditions, although more research efforts should be applied to validating the existing in vitro methods

Original language	English
Pages (from-to)	597-604
Number of pages	8
Journal	Food and Chemical Toxicology
Volume	111
DOIs	https://doi.org/10.1016/j.fct.2017.11.052
Publication status	Published - 2018 Jan

Bibliographical note

Funding Information:
This research was supported by a grant ( 15162MFDS077 ) from the Ministry of Food and Drug Safety in 2015 and a Kore University Grant ( K1604281 ). We wish to gratefully acknowledge the kind support of Professor Dr. Avrelija Cencic (deceased December 2012), in making available the PSI cell line. We, as a scientific community, will keep in highest esteem a great scientist of our time. The authors also thank the Korea University-CJ Food Safety Center (Seoul, South Korea) for providing the equipment and facilities.

Publisher Copyright:
© 2017 Elsevier Ltd

Keywords

Bioaccessibility
Cellular uptake
Cooked rice
In vitro digestion/Caco-2 cell model
Inorganic arsenic

ASJC Scopus subject areas

Food Science
Toxicology

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10.1016/j.fct.2017.11.052

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title = "Assessment of the effect of cooking on speciation and bioaccessibility/cellular uptake of arsenic in rice, using in vitro digestion and Caco-2 and PSI cells as model",

abstract = "In vitro digestion/Caco-2 or pig small intestinal epithelium cell line (PSI) uptake models were used to study the bioaccessibility and cellular uptake of arsenic (As) in cooked white rice and brown rice. The arsenite(AsIII), was the predominant species in cooked rice and in its bioaccessible fractions. The percentage of total As bioaccessibility in white rice (75%) was slightly higher (p=0.061) than that in brown rice(66%). However, there was no difference in the inorganic As (iAs) bioaccessibility between white rice (95%) and brown rice (96%). In Caco-2 cell monolayer, total As retention was 7-31%, transport was 4-25%, and uptake (sum of retention and transport) was 16-38%. In PSI cell model, the retention, transport, and uptake of tAs were 10-28%, 14-31%, and 29-50%, respectively. In both cells, the cellular uptake of tAs in brown rice was 1.4-1.5 folds lower (p<0.05) than that of white rice. These results indicate that the cellular uptake of As can be affected by nutritional compositions. These in vitro screening methods can serve as preliminary screens to predict the relative impact in rice matrix having different As species and processing conditions, although more research efforts should be applied to validating the existing in vitro methods",

keywords = "Bioaccessibility, Cellular uptake, Cooked rice, In vitro digestion/Caco-2 cell model, Inorganic arsenic",

author = "Lee, {Seul Gi} and Jinhye Kim and Hyunjoon Park and Wilhelm Holzapfel and Lee, {Kwang Won}",

note = "Funding Information: This research was supported by a grant ( 15162MFDS077 ) from the Ministry of Food and Drug Safety in 2015 and a Kore University Grant ( K1604281 ). We wish to gratefully acknowledge the kind support of Professor Dr. Avrelija Cencic (deceased December 2012), in making available the PSI cell line. We, as a scientific community, will keep in highest esteem a great scientist of our time. The authors also thank the Korea University-CJ Food Safety Center (Seoul, South Korea) for providing the equipment and facilities. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2018",

month = jan,

doi = "10.1016/j.fct.2017.11.052",

language = "English",

volume = "111",

pages = "597--604",

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AU - Lee, Seul Gi

AU - Kim, Jinhye

AU - Park, Hyunjoon

AU - Holzapfel, Wilhelm

AU - Lee, Kwang Won

N1 - Funding Information: This research was supported by a grant ( 15162MFDS077 ) from the Ministry of Food and Drug Safety in 2015 and a Kore University Grant ( K1604281 ). We wish to gratefully acknowledge the kind support of Professor Dr. Avrelija Cencic (deceased December 2012), in making available the PSI cell line. We, as a scientific community, will keep in highest esteem a great scientist of our time. The authors also thank the Korea University-CJ Food Safety Center (Seoul, South Korea) for providing the equipment and facilities. Publisher Copyright: © 2017 Elsevier Ltd

PY - 2018/1

Y1 - 2018/1

N2 - In vitro digestion/Caco-2 or pig small intestinal epithelium cell line (PSI) uptake models were used to study the bioaccessibility and cellular uptake of arsenic (As) in cooked white rice and brown rice. The arsenite(AsIII), was the predominant species in cooked rice and in its bioaccessible fractions. The percentage of total As bioaccessibility in white rice (75%) was slightly higher (p=0.061) than that in brown rice(66%). However, there was no difference in the inorganic As (iAs) bioaccessibility between white rice (95%) and brown rice (96%). In Caco-2 cell monolayer, total As retention was 7-31%, transport was 4-25%, and uptake (sum of retention and transport) was 16-38%. In PSI cell model, the retention, transport, and uptake of tAs were 10-28%, 14-31%, and 29-50%, respectively. In both cells, the cellular uptake of tAs in brown rice was 1.4-1.5 folds lower (p<0.05) than that of white rice. These results indicate that the cellular uptake of As can be affected by nutritional compositions. These in vitro screening methods can serve as preliminary screens to predict the relative impact in rice matrix having different As species and processing conditions, although more research efforts should be applied to validating the existing in vitro methods

AB - In vitro digestion/Caco-2 or pig small intestinal epithelium cell line (PSI) uptake models were used to study the bioaccessibility and cellular uptake of arsenic (As) in cooked white rice and brown rice. The arsenite(AsIII), was the predominant species in cooked rice and in its bioaccessible fractions. The percentage of total As bioaccessibility in white rice (75%) was slightly higher (p=0.061) than that in brown rice(66%). However, there was no difference in the inorganic As (iAs) bioaccessibility between white rice (95%) and brown rice (96%). In Caco-2 cell monolayer, total As retention was 7-31%, transport was 4-25%, and uptake (sum of retention and transport) was 16-38%. In PSI cell model, the retention, transport, and uptake of tAs were 10-28%, 14-31%, and 29-50%, respectively. In both cells, the cellular uptake of tAs in brown rice was 1.4-1.5 folds lower (p<0.05) than that of white rice. These results indicate that the cellular uptake of As can be affected by nutritional compositions. These in vitro screening methods can serve as preliminary screens to predict the relative impact in rice matrix having different As species and processing conditions, although more research efforts should be applied to validating the existing in vitro methods

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SN - 0278-6915

VL - 111

SP - 597

EP - 604

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

ER -

Assessment of the effect of cooking on speciation and bioaccessibility/cellular uptake of arsenic in rice, using in vitro digestion and Caco-2 and PSI cells as model

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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