Astaxanthin production by a highly photosensitive Haematococcus mutant

Min Eui Hong; Seung Phill Choi; Youn Il Park; Young Kee Kim; Won Seok Chang; Byung Woo Kim; Sang Jun Sim

doi:10.1016/j.procbio.2012.07.007

Astaxanthin production by a highly photosensitive Haematococcus mutant

Min Eui Hong
, Seung Phill Choi
, Youn Il Park
, Young Kee Kim
, Won Seok Chang
, Byung Woo Kim
, Sang Jun Sim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Haematococcus pluvialis synthesizes a high yield of astaxanthin using CO₂ in a photoautotrophic culture without contaminant heterotrophs; however, it takes too long to induce astaxanthin production. In this study, a highly photosensitive mutant strain was attained by conventional random mutagenesis and an efficient isolation method to shorten induction time. Sensitivity to photoinhibition in this mutant was raised by a partial lesion in the photosystem II (PSII) of photosynthesis, thereby prompting a change in cellular morphology as well as stimulating carotenogenesis (astaxanthin production). As a result, the concentrations of cell biomass and astaxanthin were dramatically increased by 27% and 62% under strong light and 79% and 153% under moderate light, respectively. This Haematococcus mutant would be useful for the economical astaxanthin production capable of reducing the light energy cost in a photoautotrophic culture system, even in areas with insufficient sunlight.

Original language	English
Pages (from-to)	1972-1979
Number of pages	8
Journal	Process Biochemistry
Volume	47
Issue number	12
DOIs	https://doi.org/10.1016/j.procbio.2012.07.007
Publication status	Published - 2012 Dec

Bibliographical note

Funding Information:
We acknowledge the financial support by grants from Korea CCS R&D Center and Advanced Biomass R&D Center (ABC) ( 2010-0029728 ), funded by the Ministry of Education, Science and Technology of Korean Government of the Republic of Korea . This work has also been supported by the Korea District Heating Corporation as a part of the Project of “Development of the technology for CO 2 reduction in flue gas and biodiesel production by microalgae using closed system” (1003542011S037) in “New Renewable Energy Technology R&D” project.

Keywords

Astaxanthin
Haematococcus pluvialis
Photo-inducible mutant
Photo-sensitivity

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2012.07.007

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title = "Astaxanthin production by a highly photosensitive Haematococcus mutant",

abstract = "Haematococcus pluvialis synthesizes a high yield of astaxanthin using CO2 in a photoautotrophic culture without contaminant heterotrophs; however, it takes too long to induce astaxanthin production. In this study, a highly photosensitive mutant strain was attained by conventional random mutagenesis and an efficient isolation method to shorten induction time. Sensitivity to photoinhibition in this mutant was raised by a partial lesion in the photosystem II (PSII) of photosynthesis, thereby prompting a change in cellular morphology as well as stimulating carotenogenesis (astaxanthin production). As a result, the concentrations of cell biomass and astaxanthin were dramatically increased by 27\% and 62\% under strong light and 79\% and 153\% under moderate light, respectively. This Haematococcus mutant would be useful for the economical astaxanthin production capable of reducing the light energy cost in a photoautotrophic culture system, even in areas with insufficient sunlight.",

keywords = "Astaxanthin, Haematococcus pluvialis, Photo-inducible mutant, Photo-sensitivity",

author = "Hong, \{Min Eui\} and Choi, \{Seung Phill\} and Park, \{Youn Il\} and Kim, \{Young Kee\} and Chang, \{Won Seok\} and Kim, \{Byung Woo\} and Sim, \{Sang Jun\}",

note = "Funding Information: We acknowledge the financial support by grants from Korea CCS R\&D Center and Advanced Biomass R\&D Center (ABC) ( 2010-0029728 ), funded by the Ministry of Education, Science and Technology of Korean Government of the Republic of Korea . This work has also been supported by the Korea District Heating Corporation as a part of the Project of “Development of the technology for CO 2 reduction in flue gas and biodiesel production by microalgae using closed system” (1003542011S037) in “New Renewable Energy Technology R\&D” project.",

year = "2012",

month = dec,

doi = "10.1016/j.procbio.2012.07.007",

language = "English",

volume = "47",

pages = "1972--1979",

journal = "Process Biochemistry",

issn = "1359-5113",

publisher = "Elsevier Ltd",

number = "12",

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T1 - Astaxanthin production by a highly photosensitive Haematococcus mutant

AU - Hong, Min Eui

AU - Choi, Seung Phill

AU - Park, Youn Il

AU - Kim, Young Kee

AU - Chang, Won Seok

AU - Kim, Byung Woo

AU - Sim, Sang Jun

N1 - Funding Information: We acknowledge the financial support by grants from Korea CCS R&D Center and Advanced Biomass R&D Center (ABC) ( 2010-0029728 ), funded by the Ministry of Education, Science and Technology of Korean Government of the Republic of Korea . This work has also been supported by the Korea District Heating Corporation as a part of the Project of “Development of the technology for CO 2 reduction in flue gas and biodiesel production by microalgae using closed system” (1003542011S037) in “New Renewable Energy Technology R&D” project.

PY - 2012/12

Y1 - 2012/12

N2 - Haematococcus pluvialis synthesizes a high yield of astaxanthin using CO2 in a photoautotrophic culture without contaminant heterotrophs; however, it takes too long to induce astaxanthin production. In this study, a highly photosensitive mutant strain was attained by conventional random mutagenesis and an efficient isolation method to shorten induction time. Sensitivity to photoinhibition in this mutant was raised by a partial lesion in the photosystem II (PSII) of photosynthesis, thereby prompting a change in cellular morphology as well as stimulating carotenogenesis (astaxanthin production). As a result, the concentrations of cell biomass and astaxanthin were dramatically increased by 27% and 62% under strong light and 79% and 153% under moderate light, respectively. This Haematococcus mutant would be useful for the economical astaxanthin production capable of reducing the light energy cost in a photoautotrophic culture system, even in areas with insufficient sunlight.

AB - Haematococcus pluvialis synthesizes a high yield of astaxanthin using CO2 in a photoautotrophic culture without contaminant heterotrophs; however, it takes too long to induce astaxanthin production. In this study, a highly photosensitive mutant strain was attained by conventional random mutagenesis and an efficient isolation method to shorten induction time. Sensitivity to photoinhibition in this mutant was raised by a partial lesion in the photosystem II (PSII) of photosynthesis, thereby prompting a change in cellular morphology as well as stimulating carotenogenesis (astaxanthin production). As a result, the concentrations of cell biomass and astaxanthin were dramatically increased by 27% and 62% under strong light and 79% and 153% under moderate light, respectively. This Haematococcus mutant would be useful for the economical astaxanthin production capable of reducing the light energy cost in a photoautotrophic culture system, even in areas with insufficient sunlight.

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KW - Haematococcus pluvialis

KW - Photo-inducible mutant

KW - Photo-sensitivity

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DO - 10.1016/j.procbio.2012.07.007

M3 - Article

AN - SCOPUS:84870836929

SN - 1359-5113

VL - 47

SP - 1972

EP - 1979

JO - Process Biochemistry

JF - Process Biochemistry

IS - 12

ER -

Astaxanthin production by a highly photosensitive Haematococcus mutant

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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