Aster glehni Extract Containing Caffeoylquinic Compounds Protects Human Keratinocytes through the TRPV4-PPAR δ-AMPK Pathway

Yong Jik Lee, Yoo Na Jang, Yoon Mi Han, Hyun Min Kim, Changbae Jin, Hyoung Ja Kim, Hong Seog Seo

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)


    Aster glehni (AG) has been used in cooking and as a medicine to treat various diseases for over hundreds of years in Korea. To speculate the protective effects of AG on skin barrier, we estimated the protein levels of biomarkers related to skin barrier protection in human keratinocytes, HaCaT cells treated with sodium dodecyl sulfate (SDS), or 2,4-dinitrochlorobenzene (DNCB). The protein levels for keratin, involucrin, defensin, tumor necrosis factor alpha (TNFα), peroxisome proliferator-activated receptor delta (PPARδ), 5′ adenosine monophosphate-activated protein kinase (AMPK), serine palmitoyltransferase long chain base subunit 2 (SPTLC2), and transient receptor potential cation channel subfamily V member 4 (TRPV4) were evaluated using western blotting or immunocytochemistry in HaCaT cells. AG extract increased the protein levels of PPARδ, phosphorylated AMPK, SPTLC2, keratin, involucrin, and defensin compared to the SDS or DNCB control group. However, TNFα expression increased by SDS or DNCB was decreased with AG extract. The order of action of each regulatory biomarker in AG pathway was identified TRPV4→PPARδ→AMPK from antagonist and siRNA treatment studies. AG can ameliorate the injury of keratinocytes caused by SDS or DNCB through the sequential regulation of TRPV4→PPARδ→AMPK pathway.

    Original languageEnglish
    Article number9616574
    JournalEvidence-based Complementary and Alternative Medicine
    Publication statusPublished - 2018

    Bibliographical note

    Funding Information:
    This research was supported by an intramural grant (2E28030) from the Korea Institute of Science and Technology, a grant from the National Research Foundation of Korea (NRF-2016R1A2B3013825), a grant from the Ministry of Future Creation and Science of Korea (2018K000255), a Korea University grant, a Korea University Guro Hospital grant (O1801781), and a grant from BK21 Plus Korea University Medical Science graduate program.

    Publisher Copyright:
    © 2018 Yong-Jik Lee et al.

    ASJC Scopus subject areas

    • Complementary and alternative medicine


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