Astrocytic AEG-1 regulates expression of TREK-1 under acute hypoxia

Ajung Kim, Hyun Gug Jung, Seung Chan Kim, Minji Choi, Jae Yong Park, Seok Geun Lee, Eun Mi Hwang

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


TREK-1 (TWIK-related K+ channel), a member of the two-pore domain K+ (K2P) channel family, is highly expressed in astrocytes, where it plays a key role in glutamate release and passive conductance. In addition, TREK-1 is induced to protect neurons under pathological conditions such as hypoxia. However, the upstream regulation of TREK-1 remains poorly understood. In this study, we found that AEG-1 (astrocyte elevated gene-1) regulates the expression of astrocytic TREK-1 under hypoxic conditions. Upregulation of AEG-1 increased expression of TREK-1 in astrocytes, and knockdown of AEG-1 dramatically decreased the mRNA and protein levels of TREK-1, which were restored by expression of shRNA-insensitive AEG-1. In addition, expression of TREK-1 was not regulated in the absence of AEG-1, even when HIF1α was present. Together, these results suggest that AEG-1 acts as a major upstream regulator of TREK-1 channels in astrocytes under hypoxia. Significance of the study: Previous studies have reported that hypoxia increases the expression of astrocytic TREK-1 and that increased TREK-1 expression protects neuronal cells from apoptosis. However, its cellular mechanism is not clear. In this study we first showed that AEG-1 is a major mediator of hypoxic-regulated TREK-1 expression in normal astrocytes independently of HIF-1α.

Original languageEnglish
Pages (from-to)167-175
Number of pages9
JournalCell Biochemistry and Function
Issue number2
Publication statusPublished - 2020 Mar 1

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) of Korea (NRF 2016M3C7A1904149) and Korea Institute of Science and Technology (KIST) (2E29180 and 2Z05790‐19‐039).

Publisher Copyright:
© 2019 John Wiley & Sons, Ltd.


  • AEG-1
  • Hif1α
  • TREK-1
  • astrocyte
  • hypoxia

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology


Dive into the research topics of 'Astrocytic AEG-1 regulates expression of TREK-1 under acute hypoxia'. Together they form a unique fingerprint.

Cite this