Automatic microarray image segmentation based on watershed transformation

Chang Beom Park, Kwang Woo Lee, Seong Whan Lee

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    3 Citations (Scopus)

    Abstract

    Microarrays are miniature arrays of gene fragments attached to glass chips. Microarrays allow the detection of subtle differences in genome sequences so that they can be used to detect and classify genetic diseases very accurately. Microarray experiments generate large amounts of data, because they allow thousands of genes to be processed in a single experiment. To obtain meaningful information from the massive microarray experimental results, it is needed to develop a fully automatic subgrid and spot segmentation algorithm which can measure the expression levels of each gene and the relative ratios of the genes in different situations without additional information or user intervention. In this paper, we used watershed transformation to get basic features of microarray images. Then, a graph model was used for subgrid gridding and spot segmentation based on the watershed transformation results. To verify the efficiency of our algorithm, we compared its performance with that of two previous methods: Profile and MKNN(Modified K Nearest Neighbor) algorithm. The result demonstrated the accuracy and robustness of the proposed algorithm in subgrid and spot segmentation.

    Original languageEnglish
    Title of host publicationProceedings of the 17th International Conference on Pattern Recognition, ICPR 2004
    EditorsJ. Kittler, M. Petrou, M. Nixon
    Pages786-789
    Number of pages4
    DOIs
    Publication statusPublished - 2004
    EventProceedings of the 17th International Conference on Pattern Recognition, ICPR 2004 - Cambridge, United Kingdom
    Duration: 2004 Aug 232004 Aug 26

    Publication series

    NameProceedings - International Conference on Pattern Recognition
    Volume3
    ISSN (Print)1051-4651

    Other

    OtherProceedings of the 17th International Conference on Pattern Recognition, ICPR 2004
    Country/TerritoryUnited Kingdom
    CityCambridge
    Period04/8/2304/8/26

    ASJC Scopus subject areas

    • Computer Vision and Pattern Recognition

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