Axin localizes to mitotic spindles and centrosomes in mitotic cells

Shi Mun Kim, Eun Jin Choi, Ki Joon Song, Sewoon Kim, Eunjeong Seo, Eek Hoon Jho, Sun Ho Kee

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Wnt signaling plays critical roles in cell proliferation and carcinogenesis. In addition, numerous recent studies have shown that various Wnt signaling components are involved in mitosis and chromosomal instability. However, the role of Axin, a negative regulator of Wnt signaling, in mitosis has remained unclear. Using monoclonal antibodies against Axin, we found that Axin localizes to the centrosome and along mitotic spindles. This localization was suppressed by siRNA specific for Aurora A kinase and by Aurora kinase inhibitor. Interestingly, Axin over-expression altered the subcellular distribution of Plk1 and of phosphorylated glycogen synthase kinase (GSK3β) without producing any notable changes in cellular phenotype. In the presence of Aurora kinase inhibitor, Axin over-expression induced the formation of cleavage furrow-like structures and of prominent astral microtubules lacking midbody formation in a subset of cells. Our results suggest that Axin modulates distribution of Axin-associated proteins such as Plk1 and GSK3β in an expression level-dependent manner and these interactions affect the mitotic process, including cytokinesis under certain conditions, such as in the presence of Aurora kinase inhibitor.

Original languageEnglish
Pages (from-to)943-954
Number of pages12
JournalExperimental Cell Research
Volume315
Issue number6
DOIs
Publication statusPublished - 2009 Apr 1

Bibliographical note

Funding Information:
This research was supported by a grant from the National R and D Program for Cancer Control, Ministry of Health and Welfare, Republic of Korea (Grant 0520230) to E-H. Jho and S-H. Kee, and a grant from KOSEF R21-2005-000-10017-02007 to K-J Song. S-M. Kim, E-J. Choi, S. Kim and E-J. Seo were supported by the Brain Korea 21 Program.

Keywords

  • Aurora kinase
  • Axin
  • Cytokinesis
  • Mitosis

ASJC Scopus subject areas

  • Cell Biology

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