Bacterial bioluminescent emission from recombinant Escherichia coli harboring a recA::luxCDABE fusion

Man Bock Gu, Jiho Min, Robert A. Larossa

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


This paper describes the quantitative evaluation of a bioluminescence assay for DNA damaging agents with respect to the linearity, sensitivity, specificity and dependence on the cell culture status. A recombinant bacterium, DPD2794, harboring a plasmid with a recA promoter fused to the luxCDABE operon, showed a very sensitive response to DNA-damaging stress. DPD2794 was found to show no noticeable response to non-mutagenic agents, i.e. phenol, except for some false responses appearing soon after injection. DPD2794 also showed a highly sensitive response to Mitomycin C, which was found to be a growth-stage-dependent response, not a growth-rate-dependent response. In addition, the relationship between the bioluminescence emitted in vivo, luciferase activity measured in vitro, and the amount of Lux proteins expressed was determined. The intensity of the bioluminescence emitted was found to be proportional to the luciferase activity in vitro, while the bioluminescence also seems to be correlated with the level of Lux proteins expressed in these Escherichia coli cells, up to 230 min post induction. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)45-56
Number of pages12
JournalJournal of Biochemical and Biophysical Methods
Issue number1
Publication statusPublished - 2000 Aug 10
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the Korea Ministry of Education through Korea Research Foundation (grant # 97-F-20) and supported in part by the Korea Science and Engineering Foundation (KOSEF) through ADEMRC at Kwangju Institute of Science and Technology (K-JIST). The authors are grateful for their support.


  • DNA damaging agents
  • Luciferase activity
  • SOS response
  • recA promoter

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry


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