Bacterial community composition and diversity of a full-scale integrated fixed-film activated sludge system as investigated by pyrosequencing

Soondong Kwon, Taek Seung Kim, Gi Hyeon Yu, Joon Hong Jung, Hee Deung Park

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    139 Citations (Scopus)

    Abstract

    The integrated fixed-film activated sludge (IFAS) system is a variation of the activated sludge wastewater treatment process, in which hybrid suspended and attached biomass is used to treat wastewater. Although the function and performance of the IFAS system are well studied, little is known about its microbial community structure. In this study, the composition and diversity of the bacterial community of suspended and attached biomass samples were investigated in a full-scale IFAS system using a highthroughput pyrosequencing technology. Distinct bacterial community compositions were examined for each sample and appeared to be important for its features different from conventional activated sludge processes. The abundant bacterial groups were Betaproteobacteria (59.3%), Gammaproteobacteria (8.1%), Bacteroidetes (5.2%), Alphaproteobacteria (3.9%), and Actinobacteria (3.2%) in the suspended sample, whereas Actinobacteria (14.6%), Firmicutes (13.6%), Bacteroidetes (11.6%), Betaproteobacteria (9.9%), Gammaproteobacteria (9.25%), and Alphaproteobacteria (7.4%) were major bacterial groups in the attached sample. Regarding the diversity, totals of 3,034 and 1,451 operational taxonomic units were identified at the 3% cutoff for the suspended and attached samples, respectively. Rank abundance and community analyses demonstrated that most of the diversity was originated from rare species in the samples. Taken together, the information obtained in this study will be a base for further studies relating to the microbial community structure and function of the IFAS system.

    Original languageEnglish
    Pages (from-to)1717-1723
    Number of pages7
    JournalJournal of microbiology and biotechnology
    Volume20
    Issue number12
    DOIs
    Publication statusPublished - 2010 Dec

    Keywords

    • Activated sludge
    • Community
    • Diversity
    • IFAS
    • Pyrosequencing
    • Wastewater

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

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