Abstract
The polymerase chain reaction-based Mirror Orientation Selection (MOS) method was used to isolate low temperature-induced genes from cold-treated winter barley (Hordeum vulgare L. cv. Dongbori). MOS screening identified a novel methionine (Met) aminopeptidase (MAP) designated as HvMAP. The deduced HvMAP protein was determined to possess an aminopeptidase domain and a nuclear localization signal. An in vitro enzyme assay using recombinant HvMAP protein demonstrated MAP activity. The expression of this gene was induced by low temperature and abscisic acid treatment, and overexpression of this gene conferred stronger freezing tolerance to Arabidopsis transgenic plants as compared to wild-type plants. Interestingly, low temperature treatment changed the localization of HvMAP from the nucleus to the cytoplasm. These findings suggest that HvMAP is a novel MAP that functions in freezing tolerance by facilitating protein maturation.
| Original language | English |
|---|---|
| Pages (from-to) | 53-60 |
| Number of pages | 8 |
| Journal | Plant Science |
| Volume | 180 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2011 Jan |
Bibliographical note
Funding Information:This investigation was supported by the Korea Research Foundation Grant funded by the Korean Government [KRF-2008-359-F00005] and by a grant from the BioGreen21 program by the Rural Development Administration, Republic of Korea. We thank Dr. Qi-Zhuang Ye, Chinese National Center for Drug Screening, China, for providing Met aminopeptidase substrate (Met-S-Gly-Phe).
Keywords
- Barley
- Freezing tolerance
- Low-temperature
- Methionine aminopeptidase
ASJC Scopus subject areas
- Genetics
- Agronomy and Crop Science
- Plant Science