A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.
Bibliographical noteFunding Information:
This research was supported by Basic Science Research Program and through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018M3A9H4079358) and (NRF-2019R1I1A1A01043047). This work was also supported by a Korea University Grant, NRF-2017M3D1A1039421, and KU-KIST school research fund.
© 2020 The Royal Society of Chemistry.
ASJC Scopus subject areas
- General Chemistry
- General Chemical Engineering