Bead based facile assay for sensitive quantification of native state green fluorescent protein

Jung Min Kim, Baik Lin Seong, Dong Kwon Lim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.

Original languageEnglish
Pages (from-to)13095-13099
Number of pages5
JournalRSC Advances
Volume10
Issue number22
DOIs
Publication statusPublished - 2020 Mar 31

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program and through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018M3A9H4079358) and (NRF-2019R1I1A1A01043047). This work was also supported by a Korea University Grant, NRF-2017M3D1A1039421, and KU-KIST school research fund.

Publisher Copyright:
© 2020 The Royal Society of Chemistry.

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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