Beneficial Effects of Fermentation of Red Chili Pepper Using Lactococcus lactis subs. Cremoris RPG-HL-0136 in High-Fat Diet-Induced Obese Mice

Zhi Zheng, Jung Kuk Park, Linjuan Jiang, Shaohui Zhu, Oh Wook Kwon, Byung Cheon Lee, Hea Min Lee, Yeon Jin Roh, Jae Hyun Kang, Byoung Hee Park

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Red chili pepper is a beneficial natural spicy food that has antiobesity and antitype II diabetes effects, but it is not conducive to in-depth research as a dietary strategy to treat obesity. This study aims to investigate the beneficial effects of red chili pepper, fermented with a novel Lactococcus lactis subs. cremoris RPG-HL-0136. LC-MS/MS analysis is conducted to detect the content of capsaicin and dihydrocapsaicin, and no significant difference is observed between the nonfermented red chili pepper (NFP) (W/W) and the prepared L. lactis subs. cremoris RPG-HL-0136-fermented chili mixture (LFP). After establishing a high-fat diet-induced obese type II diabetic mouse model, the effects on weight gain, weight loss of liver and testicular fat, total cholesterol, triglyceride, fasting glucose, insulin, and homeostatic model assessment for insulin resistance in LFP were evaluated to be better than those in NFP following 10 weeks of interventions. All animal experiments were approved by the Institutional Animal Care and Use Committee of Xinxiang medical university. NFP and LFP could increase the expression of transient receptor potential vanilloid subfamily 1, peroxisome proliferator-activated receptor-alpha and caspase-2 in the high-fat mice. Compared with unfermented red chili pepper, the fermented red chili pepper complex significantly reduced LPS, tumor necrosis factor-alpha, and interleukin-6 in serum (P < .05). Intake of LFP significantly increased the expression of claudin-1 and occludin in the colon of the high-fat mice (P < .05), and there was no damage to the stomach and colon. This study provides scientific evidence that red chili pepper, fermented with L. lactis subs. cremoris RPG-HL-0136, may be beneficial for future treatment of obesity and accompanying diabetes. (IACUC.No.XYLL-20200019).

Original languageEnglish
Pages (from-to)81-92
Number of pages12
JournalJournal of Medicinal Food
Issue number2
Publication statusPublished - 2023 Feb 1

Bibliographical note

Publisher Copyright:
© Copyright 2023, Mary Ann Liebert, Inc., publishers, and Korean Society of Food Science and Nutrition 2023.


  • RPG-HL-0136
  • TRPV1
  • capsaicin
  • diabetes
  • lactococcus lactis subs. cremoris
  • obesity
  • red chili pepper

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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