Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts

Hyunsoo Kim, Ick Young Kim, Soo Young Lee, Daewon Jeong

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)


In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed γ-glutamylcysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.

Original languageEnglish
Pages (from-to)5661-5665
Number of pages5
JournalFEBS Letters
Issue number24
Publication statusPublished - 2006 Oct 16

Bibliographical note

Funding Information:
This work was supported, in part, by the Molecular and Cellular BioDiscovery Research Grant (M1-0401-00-0008) from the Ministry of Science and Technology (to S.Y.L.), the Basic Research Program Grant (No. R01-2005-000-10305-0) from the KOSEF (to S.Y.L.), and the Korea Research Foundation Grant (KRF-2005-070-C00086) from the Korean government (to I.Y.K.).


  • Bone resorption
  • Glutathione
  • Osteoclast differentiation
  • Reactive oxygen species
  • Redox status

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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