Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts

Hyunsoo Kim; Ick Young Kim; Soo Young Lee; Daewon Jeong

doi:10.1016/j.febslet.2006.09.015

Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts

Hyunsoo Kim, Ick Young Kim, Soo Young Lee, Daewon Jeong

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed γ-glutamylcysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.

Original language	English
Pages (from-to)	5661-5665
Number of pages	5
Journal	FEBS Letters
Volume	580
Issue number	24
DOIs	https://doi.org/10.1016/j.febslet.2006.09.015
Publication status	Published - 2006 Oct 16

Bibliographical note

Funding Information:
This work was supported, in part, by the Molecular and Cellular BioDiscovery Research Grant (M1-0401-00-0008) from the Ministry of Science and Technology (to S.Y.L.), the Basic Research Program Grant (No. R01-2005-000-10305-0) from the KOSEF (to S.Y.L.), and the Korea Research Foundation Grant (KRF-2005-070-C00086) from the Korean government (to I.Y.K.).

Keywords

Bone resorption
Glutathione
Osteoclast differentiation
Reactive oxygen species
Redox status

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2006.09.015

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title = "Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts",

abstract = "In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed γ-glutamylcysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.",

keywords = "Bone resorption, Glutathione, Osteoclast differentiation, Reactive oxygen species, Redox status",

author = "Hyunsoo Kim and Kim, {Ick Young} and Lee, {Soo Young} and Daewon Jeong",

note = "Funding Information: This work was supported, in part, by the Molecular and Cellular BioDiscovery Research Grant (M1-0401-00-0008) from the Ministry of Science and Technology (to S.Y.L.), the Basic Research Program Grant (No. R01-2005-000-10305-0) from the KOSEF (to S.Y.L.), and the Korea Research Foundation Grant (KRF-2005-070-C00086) from the Korean government (to I.Y.K.).",

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doi = "10.1016/j.febslet.2006.09.015",

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volume = "580",

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T1 - Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts

AU - Kim, Hyunsoo

AU - Kim, Ick Young

AU - Lee, Soo Young

AU - Jeong, Daewon

N1 - Funding Information: This work was supported, in part, by the Molecular and Cellular BioDiscovery Research Grant (M1-0401-00-0008) from the Ministry of Science and Technology (to S.Y.L.), the Basic Research Program Grant (No. R01-2005-000-10305-0) from the KOSEF (to S.Y.L.), and the Korea Research Foundation Grant (KRF-2005-070-C00086) from the Korean government (to I.Y.K.).

PY - 2006/10/16

Y1 - 2006/10/16

N2 - In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed γ-glutamylcysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.

AB - In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed γ-glutamylcysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.

KW - Bone resorption

KW - Glutathione

KW - Osteoclast differentiation

KW - Reactive oxygen species

KW - Redox status

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DO - 10.1016/j.febslet.2006.09.015

M3 - Article

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AN - SCOPUS:33749534719

SN - 0014-5793

VL - 580

SP - 5661

EP - 5665

JO - FEBS Letters

JF - FEBS Letters

IS - 24

ER -

Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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