Bioaccessibility of AhR-active PAHs in sediments contaminated by the Hebei Spirit oil spill: Application of Tenax extraction in effect-directed analysis

Seongjin Hong; Un Hyuk Yim; Sung Yong Ha; Won Joon Shim; Seungyeon Jeon; Sangwoo Lee; Cheolmin Kim; Kyungho Choi; Jinho Jung; John P. Giesy; Jong Seong Khim

doi:10.1016/j.chemosphere.2015.09.043

Bioaccessibility of AhR-active PAHs in sediments contaminated by the Hebei Spirit oil spill: Application of Tenax extraction in effect-directed analysis

Seongjin Hong, Un Hyuk Yim, Sung Yong Ha, Won Joon Shim, Seungyeon Jeon, Sangwoo Lee, Cheolmin Kim, Kyungho Choi, Jinho Jung, John P. Giesy, Jong Seong Khim

Division of Environmental Science and Ecological Engineering

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Bioaccessibility of toxic substances in sedimentary residual oil is a crucial factor that needs to be considered for accurate risk assessments posed by oil spills. However, information on oil weathering processes and bioaccessibility of residual oil is often not sufficient and clear. In the present study, bioaccessibility of aryl hydrocarbon receptor (AhR)-active polycyclic aromatic hydrocarbons (PAHs) in coastal sediments near the site of the Hebei Spirit oil spill (Korea, 2007) was assessed by Tenax extraction in effect-directed analysis (EDA). Sediment samples collected 6 years after the oil spill were extracted using Soxhlet or Tenax, and EDA was performed using a battery of H4IIE-luc bioassay and GC/MSD analysis. Concentrations of PAHs and alkyl-PAHs in Soxhlet extracts ranged from 210 to 53,000 μg kg^-1 dry mass. However, concentrations of PAHs and alkyl-PAHs in Tenax extracts were approximately 20-fold less compared to those in Soxhlet extracts. In Soxhlet and Tenax extracts, the major AhR-active PAHs were identified as C1-chrysene, C3-chrysene, and C4-phenanthrene. Concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TEQ_PAHs) explained 31% and 60% of the bioassay-derived TCDD-EQ concentrations in Soxhlet and Tenax extracts, respectively. Overall, bioaccessibility of PAHs and alkyl-PAHs in sedimentary residual oils depended on hydrophobicity (log K_ow) and degree of weathering of crude oil. The results of the present study provide further evidence in support of the biological and ecological recoveries of oil spill sites.

Original language	English
Pages (from-to)	706-712
Number of pages	7
Journal	Chemosphere
Volume	144
DOIs	https://doi.org/10.1016/j.chemosphere.2015.09.043
Publication status	Published - 2016 Feb 1

Bibliographical note

Funding Information:
This work was supported by the projects entitled “Oil spill Environmental Impact Assessment and Environmental Restoration (PM56951)”, “Development of Integrated Estuarine Management System”, and “Development of Techniques for Assessment and Management of Hazardous Chemicals in the Marine Environment” funded by the Ministry of Oceans and Fisheries of Korea given to Prof. JSK. Prof. JPG was supported by the Canada Research Chair program , a Visiting Distinguished Professorship in the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong , the 2012 ″High Level Foreign Experts” (#GDW20123200120) program, funded by the State Administration of Foreign Experts Affairs , the P.R. China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences .

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

GC/MSD
H4IIE-luc bioassay
Potency balance
Sediment
Toxicity equivalents
Yellow sea

ASJC Scopus subject areas

Environmental Engineering
Environmental Chemistry
Chemistry(all)
Pollution
Health, Toxicology and Mutagenesis

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10.1016/j.chemosphere.2015.09.043

Cite this

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title = "Bioaccessibility of AhR-active PAHs in sediments contaminated by the Hebei Spirit oil spill: Application of Tenax extraction in effect-directed analysis",

abstract = "Bioaccessibility of toxic substances in sedimentary residual oil is a crucial factor that needs to be considered for accurate risk assessments posed by oil spills. However, information on oil weathering processes and bioaccessibility of residual oil is often not sufficient and clear. In the present study, bioaccessibility of aryl hydrocarbon receptor (AhR)-active polycyclic aromatic hydrocarbons (PAHs) in coastal sediments near the site of the Hebei Spirit oil spill (Korea, 2007) was assessed by Tenax extraction in effect-directed analysis (EDA). Sediment samples collected 6 years after the oil spill were extracted using Soxhlet or Tenax, and EDA was performed using a battery of H4IIE-luc bioassay and GC/MSD analysis. Concentrations of PAHs and alkyl-PAHs in Soxhlet extracts ranged from 210 to 53,000 μg kg-1 dry mass. However, concentrations of PAHs and alkyl-PAHs in Tenax extracts were approximately 20-fold less compared to those in Soxhlet extracts. In Soxhlet and Tenax extracts, the major AhR-active PAHs were identified as C1-chrysene, C3-chrysene, and C4-phenanthrene. Concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TEQPAHs) explained 31% and 60% of the bioassay-derived TCDD-EQ concentrations in Soxhlet and Tenax extracts, respectively. Overall, bioaccessibility of PAHs and alkyl-PAHs in sedimentary residual oils depended on hydrophobicity (log Kow) and degree of weathering of crude oil. The results of the present study provide further evidence in support of the biological and ecological recoveries of oil spill sites.",

keywords = "GC/MSD, H4IIE-luc bioassay, Potency balance, Sediment, Toxicity equivalents, Yellow sea",

author = "Seongjin Hong and Yim, {Un Hyuk} and Ha, {Sung Yong} and Shim, {Won Joon} and Seungyeon Jeon and Sangwoo Lee and Cheolmin Kim and Kyungho Choi and Jinho Jung and Giesy, {John P.} and Khim, {Jong Seong}",

note = "Funding Information: This work was supported by the projects entitled “Oil spill Environmental Impact Assessment and Environmental Restoration (PM56951)”, “Development of Integrated Estuarine Management System”, and “Development of Techniques for Assessment and Management of Hazardous Chemicals in the Marine Environment” funded by the Ministry of Oceans and Fisheries of Korea given to Prof. JSK. Prof. JPG was supported by the Canada Research Chair program , a Visiting Distinguished Professorship in the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong , the 2012 ″High Level Foreign Experts” (#GDW20123200120) program, funded by the State Administration of Foreign Experts Affairs , the P.R. China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences . Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

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T2 - Application of Tenax extraction in effect-directed analysis

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AU - Yim, Un Hyuk

AU - Ha, Sung Yong

AU - Shim, Won Joon

AU - Jeon, Seungyeon

AU - Lee, Sangwoo

AU - Kim, Cheolmin

AU - Choi, Kyungho

AU - Jung, Jinho

AU - Giesy, John P.

AU - Khim, Jong Seong

N1 - Funding Information: This work was supported by the projects entitled “Oil spill Environmental Impact Assessment and Environmental Restoration (PM56951)”, “Development of Integrated Estuarine Management System”, and “Development of Techniques for Assessment and Management of Hazardous Chemicals in the Marine Environment” funded by the Ministry of Oceans and Fisheries of Korea given to Prof. JSK. Prof. JPG was supported by the Canada Research Chair program , a Visiting Distinguished Professorship in the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong , the 2012 ″High Level Foreign Experts” (#GDW20123200120) program, funded by the State Administration of Foreign Experts Affairs , the P.R. China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences . Publisher Copyright: © 2015 Elsevier Ltd.

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N2 - Bioaccessibility of toxic substances in sedimentary residual oil is a crucial factor that needs to be considered for accurate risk assessments posed by oil spills. However, information on oil weathering processes and bioaccessibility of residual oil is often not sufficient and clear. In the present study, bioaccessibility of aryl hydrocarbon receptor (AhR)-active polycyclic aromatic hydrocarbons (PAHs) in coastal sediments near the site of the Hebei Spirit oil spill (Korea, 2007) was assessed by Tenax extraction in effect-directed analysis (EDA). Sediment samples collected 6 years after the oil spill were extracted using Soxhlet or Tenax, and EDA was performed using a battery of H4IIE-luc bioassay and GC/MSD analysis. Concentrations of PAHs and alkyl-PAHs in Soxhlet extracts ranged from 210 to 53,000 μg kg-1 dry mass. However, concentrations of PAHs and alkyl-PAHs in Tenax extracts were approximately 20-fold less compared to those in Soxhlet extracts. In Soxhlet and Tenax extracts, the major AhR-active PAHs were identified as C1-chrysene, C3-chrysene, and C4-phenanthrene. Concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TEQPAHs) explained 31% and 60% of the bioassay-derived TCDD-EQ concentrations in Soxhlet and Tenax extracts, respectively. Overall, bioaccessibility of PAHs and alkyl-PAHs in sedimentary residual oils depended on hydrophobicity (log Kow) and degree of weathering of crude oil. The results of the present study provide further evidence in support of the biological and ecological recoveries of oil spill sites.

AB - Bioaccessibility of toxic substances in sedimentary residual oil is a crucial factor that needs to be considered for accurate risk assessments posed by oil spills. However, information on oil weathering processes and bioaccessibility of residual oil is often not sufficient and clear. In the present study, bioaccessibility of aryl hydrocarbon receptor (AhR)-active polycyclic aromatic hydrocarbons (PAHs) in coastal sediments near the site of the Hebei Spirit oil spill (Korea, 2007) was assessed by Tenax extraction in effect-directed analysis (EDA). Sediment samples collected 6 years after the oil spill were extracted using Soxhlet or Tenax, and EDA was performed using a battery of H4IIE-luc bioassay and GC/MSD analysis. Concentrations of PAHs and alkyl-PAHs in Soxhlet extracts ranged from 210 to 53,000 μg kg-1 dry mass. However, concentrations of PAHs and alkyl-PAHs in Tenax extracts were approximately 20-fold less compared to those in Soxhlet extracts. In Soxhlet and Tenax extracts, the major AhR-active PAHs were identified as C1-chrysene, C3-chrysene, and C4-phenanthrene. Concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TEQPAHs) explained 31% and 60% of the bioassay-derived TCDD-EQ concentrations in Soxhlet and Tenax extracts, respectively. Overall, bioaccessibility of PAHs and alkyl-PAHs in sedimentary residual oils depended on hydrophobicity (log Kow) and degree of weathering of crude oil. The results of the present study provide further evidence in support of the biological and ecological recoveries of oil spill sites.

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KW - H4IIE-luc bioassay

KW - Potency balance

KW - Sediment

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Bioaccessibility of AhR-active PAHs in sediments contaminated by the Hebei Spirit oil spill: Application of Tenax extraction in effect-directed analysis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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