TY - JOUR
T1 - Biochar affects the dissipation of antibiotics and abundance of antibiotic resistance genes in pig manure
AU - Ngigi, Anastasiah N.
AU - Ok, Yong Sik
AU - Thiele-Bruhn, Sören
N1 - Funding Information:
This work was supported by German Academic Exchange Service (DAAD) through a Postdoctoral Fellowship. Laboratory materials and equipment were provided by Department of Soil Science, Trier University, Trier, Germany. Thanks go to Mrs. E. Sieberger and P. Ziegler for assistance in the laboratory.
Funding Information:
This work was supported by German Academic Exchange Service (DAAD) through a Postdoctoral Fellowship. Laboratory materials and equipment were provided by Department of Soil Science, Trier University, Trier, Germany. Thanks go to Mrs. E. Sieberger and P. Ziegler for assistance in the laboratory.
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/11
Y1 - 2020/11
N2 - Manure from medicated livestock contains pharmaceutical antibiotics and antibiotic resistance genes (ARGs). Bioavailable antibiotics trigger further ARGs amplification during manure storage. It was tested whether biochar lowers the bioavailability of the antibiotics sulfamethazine (SMZ), ciprofloxacin (CIP), oxytetracycline (OTC) and florfenicol (FF) in manure and the amplification of sul1 and tet(W) ARGs. To that end, liquid pig manure was treated with 5% (w/w) pinecone biochar (BCP). Antibiotics dissipated during 30-d incubation in the order SMZ < OTC < CIP < FF. Added BCP further immobilized SMZ, OTC and CIP, while the effect was not significant for FF. Both sul1 and tet(W) ARGs copy numbers significantly increased by factors of 5.8 and 2.5, respectively, in OTC and SMZ spiked manure. The abundance of sul1 was significantly decreased in BCP amended manure, while the impact on tet(W) was less. Consequently, biochar is suitable for the management of antibiotics contaminated manure during storage.
AB - Manure from medicated livestock contains pharmaceutical antibiotics and antibiotic resistance genes (ARGs). Bioavailable antibiotics trigger further ARGs amplification during manure storage. It was tested whether biochar lowers the bioavailability of the antibiotics sulfamethazine (SMZ), ciprofloxacin (CIP), oxytetracycline (OTC) and florfenicol (FF) in manure and the amplification of sul1 and tet(W) ARGs. To that end, liquid pig manure was treated with 5% (w/w) pinecone biochar (BCP). Antibiotics dissipated during 30-d incubation in the order SMZ < OTC < CIP < FF. Added BCP further immobilized SMZ, OTC and CIP, while the effect was not significant for FF. Both sul1 and tet(W) ARGs copy numbers significantly increased by factors of 5.8 and 2.5, respectively, in OTC and SMZ spiked manure. The abundance of sul1 was significantly decreased in BCP amended manure, while the impact on tet(W) was less. Consequently, biochar is suitable for the management of antibiotics contaminated manure during storage.
KW - ARG amplification
KW - Bioavailability
KW - Dissipation kinetics
KW - Manure storage
KW - Pig slurry
KW - Safe management
UR - http://www.scopus.com/inward/record.url?scp=85087505136&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2020.123782
DO - 10.1016/j.biortech.2020.123782
M3 - Article
C2 - 32652440
AN - SCOPUS:85087505136
SN - 0960-8524
VL - 315
JO - Bioresource technology
JF - Bioresource technology
M1 - 123782
ER -
