Biochar affects the dissipation of antibiotics and abundance of antibiotic resistance genes in pig manure

Anastasiah N. Ngigi; Yong Sik Ok; Sören Thiele-Bruhn

doi:10.1016/j.biortech.2020.123782

Biochar affects the dissipation of antibiotics and abundance of antibiotic resistance genes in pig manure

Anastasiah N. Ngigi, Yong Sik Ok, Sören Thiele-Bruhn

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Manure from medicated livestock contains pharmaceutical antibiotics and antibiotic resistance genes (ARGs). Bioavailable antibiotics trigger further ARGs amplification during manure storage. It was tested whether biochar lowers the bioavailability of the antibiotics sulfamethazine (SMZ), ciprofloxacin (CIP), oxytetracycline (OTC) and florfenicol (FF) in manure and the amplification of sul1 and tet(W) ARGs. To that end, liquid pig manure was treated with 5% (w/w) pinecone biochar (BCP). Antibiotics dissipated during 30-d incubation in the order SMZ < OTC < CIP < FF. Added BCP further immobilized SMZ, OTC and CIP, while the effect was not significant for FF. Both sul1 and tet(W) ARGs copy numbers significantly increased by factors of 5.8 and 2.5, respectively, in OTC and SMZ spiked manure. The abundance of sul1 was significantly decreased in BCP amended manure, while the impact on tet(W) was less. Consequently, biochar is suitable for the management of antibiotics contaminated manure during storage.

Original language	English
Article number	123782
Journal	Bioresource technology
Volume	315
DOIs	https://doi.org/10.1016/j.biortech.2020.123782
Publication status	Published - 2020 Nov

Bibliographical note

Funding Information:
This work was supported by German Academic Exchange Service (DAAD) through a Postdoctoral Fellowship. Laboratory materials and equipment were provided by Department of Soil Science, Trier University, Trier, Germany. Thanks go to Mrs. E. Sieberger and P. Ziegler for assistance in the laboratory.

Funding Information:
This work was supported by German Academic Exchange Service (DAAD) through a Postdoctoral Fellowship. Laboratory materials and equipment were provided by Department of Soil Science, Trier University, Trier, Germany. Thanks go to Mrs. E. Sieberger and P. Ziegler for assistance in the laboratory.

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

ARG amplification
Bioavailability
Dissipation kinetics
Manure storage
Pig slurry
Safe management

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2020.123782

Cite this

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title = "Biochar affects the dissipation of antibiotics and abundance of antibiotic resistance genes in pig manure",

abstract = "Manure from medicated livestock contains pharmaceutical antibiotics and antibiotic resistance genes (ARGs). Bioavailable antibiotics trigger further ARGs amplification during manure storage. It was tested whether biochar lowers the bioavailability of the antibiotics sulfamethazine (SMZ), ciprofloxacin (CIP), oxytetracycline (OTC) and florfenicol (FF) in manure and the amplification of sul1 and tet(W) ARGs. To that end, liquid pig manure was treated with 5% (w/w) pinecone biochar (BCP). Antibiotics dissipated during 30-d incubation in the order SMZ < OTC < CIP < FF. Added BCP further immobilized SMZ, OTC and CIP, while the effect was not significant for FF. Both sul1 and tet(W) ARGs copy numbers significantly increased by factors of 5.8 and 2.5, respectively, in OTC and SMZ spiked manure. The abundance of sul1 was significantly decreased in BCP amended manure, while the impact on tet(W) was less. Consequently, biochar is suitable for the management of antibiotics contaminated manure during storage.",

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note = "Funding Information: This work was supported by German Academic Exchange Service (DAAD) through a Postdoctoral Fellowship. Laboratory materials and equipment were provided by Department of Soil Science, Trier University, Trier, Germany. Thanks go to Mrs. E. Sieberger and P. Ziegler for assistance in the laboratory. Funding Information: This work was supported by German Academic Exchange Service (DAAD) through a Postdoctoral Fellowship. Laboratory materials and equipment were provided by Department of Soil Science, Trier University, Trier, Germany. Thanks go to Mrs. E. Sieberger and P. Ziegler for assistance in the laboratory. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

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Biochar affects the dissipation of antibiotics and abundance of antibiotic resistance genes in pig manure

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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