Biochemical analysis of Escherichia coli selenophosphate synthetase mutants. Lysine 20 is essential for catalytic activity and cysteine 17/19 for 8-azido-ATP derivatization

Medicine & Life Sciences

• 8-azidoadenosine 5'-triphosphate 93%
• Adenosine Triphosphate 40%
• Binding Sites 4%
• Cysteine 49%
• Enzymes 12%
• Escherichia coli 36%
• Gel Chromatography 5%
• Glycine 5%
• Guanosine Triphosphate 6%
• Lysine 50%
• Mutation 12%
• Peptides 3%
• Proteins 6%
• Selenium Compounds 9%
• selenophosphate 11%
• selenophosphate synthetase 100%
• Serine 5%
• Site-Directed Mutagenesis 6%

Chemical Compounds

• Cysteine 44%
• Cysteine Residue 6%
• Donor 4%
• Lysine 49%
• Mutation 21%
• Peptide 3%
• Photoaffinity Labelling 8%
• Protein 8%
• Selenide 7%
• Selenophosphate 91%
• Serine 6%
• Size Exclusion Chromatography 5%