Bioconversion of cellulose into ethanol by nonisothermal simultaneous saccharification and fermentation

Kyeong Keun Oh, Seung Wook Kim, Yong Seob Jeong, Suk In Hong

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)


The kinetic characteristics of cellulase and β-glucosidase during hydrolysis were determined. The kinetic parameters were found to reproduce experimental data satisfactorily and could be used in a simultaneous saccharification and fermentation (SSF) system by coupling with a fermentation model. The effects of temperature on yeast growth and ethanol production were investigated in batch cultures. In the range of 35-45°C, using a mathematical model and a computer simulation package, the kinetic parameters at each temperature were estimated. The appropriate forms of the model equation for the SSF considering the effects of temperature were developed, and the temperature profile for maximizing the ethanol production was also obtained. Briefly, the optimum temperature profile began at a low temperature of 35°C, which allows the propagation of cells. Up to 10 h, the operating temperature increased rapidly to 39°C, and then decreased slowly to 36°C. In this nonisothermal SSF system with the above temperature profile, a maximum ethanol production of 14.87 g/L was obtained.

Original languageEnglish
Pages (from-to)15-30
Number of pages16
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Issue number1
Publication statusPublished - 2000


  • Kinetic modeling
  • Nonisothermal simultaneous saccharification and fermentation
  • Temperature profile

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology


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